Various lysates were subjected to SDS PAGE followed by western blot with 28347-1-AP (EEA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB result of EEA1 antibody (28347-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (28347-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28347-1-AP (EEA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28347-1-AP (EEA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).