Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60293 (OPTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-0 (HRP-conjugated Streptavidin) as secondary antibody, at dilution of 1:3000 (lane 1 and 3) or 1:6000 (lane 2 and 4).
IP Result of anti-EBAG9 (IP:12255-1-AP, 3ug; Detection:12255-1-AP 1:800) with mouse heart tissue lysate. SA00001-18 (HRP-conjugated Protein A from S.aureus) as secondary antibody.
Various lysates were subjected to SDS PAGE followed by western blot with 30332-1-AP (ARL13B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. SA00001-12 (HRP-conjugated Goat Anti-Guinea pig IgG(H+L) ) as the secondary antibody.
