Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP result of anti-Hexokinase 1 (IP:19662-1-AP, 4ug; Detection:19662-1-AP 1:2000) with HeLa cells lysate 1720 ug.