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54 product results for "IFNg"

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HumanKine® recombinant human IFN gamma protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

26 Publications

Reviews (1)

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Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.02-0.14 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat, pig

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1301
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Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IFN gamma (HZ-1301) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

Proteintech IFN gamma (HZ-1301) demonstrates a 3-fold lower EC50 and equivalent dose-dependent inhibition of proliferation to a leading competitor. Recombinant human IFN gamma dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.
HumanKine® recombinant human IFN gamma protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.02-0.14 ng/mL

Expression

HEK293

Reactivity

human

Purity

> 95%

Sizes
-_- / -_-

Cat No. HZ-1301-GMP
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Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IFN gamma (HZ-1301-GMP) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of GMP recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.02-0.14 ng/mL.
IFN-gamma Polyclonal antibody

KO/KD Validated

107 Publications

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PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, rat, pig

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 15365-1-AP
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PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IFN-gamma Recombinant antibodies (241390B10) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

241390B10
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Cat No. 98187-1-PBS, 98187-1-RR
Anti-Mouse IFN gamma (XMG1.2) antibodies (5 total products)

7 Publications

Host/IsoType

Rat/IgG1, kappa

Reactivity

mouse

Applications

FC (Intra)

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, FITC Plus, PE, Unconjugated

CloneNo.

XMG1.2
-_- / -_-

Cat No. 65153-1-Ig, FITC-65153, PE-65153, CL488-65153, CL647-65153
AuthentiKine™ Human IFN-gamma ELISA Kit

21 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture superntants

Sensitivity

0.4 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE00146
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IFN-gamma Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP01131-1	84246-3-PBS	84246-2-PBS	IFN-gamma	Human	Cytometric bead array, Sandwich ELISA	sELISA: 7.80-500 pg/mL, CBA: 0.625-80 ng/mL
MP00498-1	82768-6-PBS	82768-4-PBS	IFN-gamma	Mouse	Cytometric bead array	CBA: 0.78-100 ng/mL
MP00498-2	82768-5-PBS	82768-4-PBS	IFN-gamma	Mouse	Cytometric bead array	CBA: 0.78-100 ng/mL
MP01131-2	84246-1-PBS	84246-2-PBS	IFN-gamma	Human	Cytometric bead array	CBA: 0.625-40 ng/mL
MP50092-1	68739-1-PBS	68739-2-PBS	IFN-gamma	Human	Sandwich ELISA	sELISA: 0.977-15.625 ng/mL

Mouse IFN-gamma ELISA Kit

8 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

0.16 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE10094
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Rat IFN-gamma ELISA Kit

2 Publications

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Assay Type

Sandwich

Sample Type

Cell culture supernatants

Sensitivity

3.5 pg/mL

Range

125-8000 pg/mL

Sizes
-_- / -_-

Cat No. KE20007
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IFN-gamma Polyclonal antibody

1 Publications

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Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Host/IsoType

Rabbit/IgG

Reactivity

rat

Applications

IHC, FC (Intra), ELISA

Sizes
-_- / -_-

Cat No. 30293-1-AP
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IFN Gamma (30293-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35), and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
NeutraKine® IFN Gamma Monoclonal antibodies (1E10G7) (4 total products)

1 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

FC (Intra), Neutralization & Neutro-controls

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, Unconjugated

CloneNo.

1E10G7
-_- / -_-

Cat No. 69007-1-Ig, CL647-69007, 69007-1-PBS, CL488-69007
anti-IFNy recombinant VHH, biotinylated

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BLI analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS).

Application

BLI, SPR, ELISA

Conjugate

Biotin

Reactivity

human

Sizes
-_- / -_-

Cat No. IFNg-b
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BLI analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS).

ELISA analysis of the capture of varying concentrations of HumanKine Interferon γ (HZ-1301) by biotinylated anti-INFg VHH (IFNgNHS). Detection via rabbit polyclonal PTG 15365-1-AP.
anti-IFNy recombinant VHH, for NHS conjugation

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BLI analysis of the interaction between varying concentrations of anti-IFNg VHH (IFNgNHS) and immobilised, biotinylated HumanKine Interferon γ (HZ-1301). Fit indicates fitting of data to a 1:1 binding model.

Application

Conjugation

Conjugate

Unconjugated

Reactivity

human

Sizes
-_- / -_-

Cat No. IFNgNHS
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BLI analysis of the interaction between varying concentrations of anti-IFNg VHH (IFNgNHS) and immobilised, biotinylated HumanKine Interferon γ (HZ-1301). Fit indicates fitting of data to a 1:1 binding model.
IFN-gamma Polyclonal antibody

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PMA, Ionomycin and Brefeldin A treated CTLL-2 cells were subjected to SDS PAGE followed by western blot with 29788-1-AP (IFN gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 29788-1-AP
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PMA, Ionomycin and Brefeldin A treated CTLL-2 cells were subjected to SDS PAGE followed by western blot with 29788-1-AP (IFN gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IFN-gamma Polyclonal antibody

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Non-treated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 28722-1-AP (IFNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 28722-1-AP
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Non-treated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 28722-1-AP (IFNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IFN-gamma Recombinant antibodies (240262C9) (6 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugation

CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 647, CoraLite® Plus 750, Unconjugated

CloneNo.

240262C9
-_- / -_-

Cat No. CL750-98045, CL405-98045, CL647-98045, 98045-1-PBS, CL488-98045, 98045-1-RR
IFN-gamma Recombinant antibodies (6K20) (5 total products)

Host/IsoType

Rabbit/IgG

Reactivity

rat

Applications

FC (Intra)

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, PE, Unconjugated

CloneNo.

6K20
-_- / -_-

Cat No. CL488-98001, PE-98001, 98001-1-PBS, CL647-98001, 98001-1-RR
MultiPro® 5CFLX Anti-Human IFN Gamma (1E10G7)

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G69007-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

1E10G7

Sizes
-_- / -_-

Cat No. G69007-1-5C
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G69007-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G69007-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G69007-1-5C was used to stain PBMC under 4hr PMA + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.
NeutraControl IFN-gamma Monoclonal antibody

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Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Non-Neutralization

CloneNo.

2F11G6

Sizes
-_- / -_-

Cat No. 69507-1-Ig
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Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
Rat IFN-gamma ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

9.5 pg/mL

Range

62.5-4000 pg/mL

Sizes
-_- / -_-

Cat No. KE20020
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IFNG E.Coli expressed Tagged-proteins (5 total products)

2 Publications

Species

human, mouse

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag30395, Ag8321, Ag23462, Ag23466, Ag8320