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74 product results for "IL-1 Beta"

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HumanKine® recombinant human IL-1 beta protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

41 Publications

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Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

Typically ≤ 0.05 ng/mL in D10.G4.1 cells

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1164
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Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.  

Recombinant human IL-1 beta (Cat no: HZ-1164) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.8-4.0 ng/mL ng/mL EC50. 
IL-1 Beta Polyclonal antibody

KO/KD Validated

720 Publications

Reviews (2)

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Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, canine, pig, rabbit, sheep, zebrafish

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 16806-1-AP
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Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IL-1 beta Recombinant antibodies (240902D6) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, ELISA, IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

240902D6
-_- / -_-

Cat No. 82696-15-PBS, 82696-15-RR
IL-1 Beta Monoclonal antibodies (2A1B4) (2 total products)

KO/KD Validated

80 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, bovine, rabbit

Applications

ELISA, IF/ICC, IHC, IP, WB

Conjugation

Unconjugated

CloneNo.

2A1B4
-_- / -_-

Cat No. 66737-1-PBS, 66737-1-Ig
Mouse IL-1 beta ELISA Kit

246 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants, tissue lysates

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE10003
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IL-1 beta Polyclonal antibody

125 Publications

Reviews (1)

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LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 26048-1-AP
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LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Human IL-1 beta ELISA Kit

95 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1.5 pg/mL

Range

3.9-250 pg/mL

Sizes
-_- / -_-

Cat No. KE00021
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IL-1 beta Recombinant antibodies (240902G6) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

240902G6
-_- / -_-

Cat No. 82696-17-PBS, 82696-17-RR
IL-1 beta Recombinant antibodies (241364D5) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

241364D5
-_- / -_-

Cat No. 84136-5-RR, 84136-5-PBS
IL-1 Beta Recombinant antibodies (3I9) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, ELISA, WB

Conjugation

Unconjugated

CloneNo.

3I9
-_- / -_-

Cat No. 82696-6-PBS, 82696-6-RR

IL-1 beta Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00331-2	82696-15-PBS	82696-8-PBS	IL-1 beta	Human	Cytometric bead array, Sandwich ELISA	sELISA: 7.8-500 pg/mL, CBA: 0.313-40 ng/mL
MP00331-1	82696-7-PBS	82696-5-PBS	IL-1 beta	Human	Cytometric bead array	CBA: 0.781-100 ng/mL
MP00331-3	82696-15-PBS	82696-12-PBS	IL-1 beta	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00331-4	82696-16-PBS	82696-11-PBS	IL-1 beta	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP01046-1	84136-2-PBS	84136-3-PBS	IL-1 beta	Mouse	Cytometric bead array	CBA: 0.313-5 ng/mL
MP01046-2	84136-2-PBS	84136-4-PBS	IL-1 beta	Mouse	Cytometric bead array	CBA: 0.313-10 ng/mL
MP01046-3	84136-1-PBS	84136-3-PBS	IL-1 beta	Mouse	Cytometric bead array	CBA: 0.313-20 ng/mL
MP01210-1	84274-2-PBS	84274-3-PBS	IL-1 beta	Rat	Cytometric bead array	CBA: 0.313-40 ng/mL
MP01210-2	84274-2-PBS	84274-4-PBS	IL-1 beta	Rat	Cytometric bead array	CBA: 0.313-40 ng/mL
MP01210-3	84274-2-PBS	84274-1-PBS	IL-1 beta	Rat	Cytometric bead array	CBA: 0.313-40 ng/mL
MP50134-1	66737-2-PBS	68767-1-PBS	IL-1 beta	Human	Sandwich ELISA	sELISA: 1.563-100 ng/mL
MP80001-1	82696-6-PBS	65276-1-PBS	IL-1 beta	Human	Cytometric bead array	CBA: 6.25-100 ng/mL
MP80001-2	82696-6-PBS	68767-1-PBS	IL-1 beta	Human	Cytometric bead array	CBA: 3.125-100 ng/mL
MP80001-3	82696-3-PBS	68767-2-PBS	IL-1 beta	Human	Cytometric bead array	CBA: 1.563-100 ng/mL
MP80001-4	82696-5-PBS	68767-2-PBS	IL1B	Human	Cytometric bead array	CBA: 3.125-100 ng/mL
MP80001-5	82696-3-PBS	65276-1-PBS	IL1B	Human	Cytometric bead array	CBA: 3.125-100 ng/mL
MP80001-6	82696-8-PBS	66737-1-PBS	IL-1 beta	Human	Sandwich ELISA	sELISA: 0.098-12.5 ng/mL

Anti-Human IL-1 beta (2A7C6) antibodies (6 total products)

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

Cytometric bead array, ELISA, FC (Intra)

Conjugation

CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 647, CoraLite® Plus 750, PE, Unconjugated

CloneNo.

2A7C6
-_- / -_-

Cat No. PE-65276, CL647-65276, 65276-1-PBS, CL405-65276, CL750-65276, CL488-65276
HumanKine® recombinant human IL-1 beta protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

View More Images (2)

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Purity of GMP recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.8-4.0 ng/mL in an HEK293 reporter cell line

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1164-GMP
×

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Purity of GMP recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-1 beta (Cat no: HZ-1164-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.8-4.0 ng/mL EC50. 
IL-1 Beta Polyclonal antibody

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LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30342-1-AP (IL-1 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 30342-1-AP
×

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LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30342-1-AP (IL-1 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IL-1 beta Polyclonal antibody

Enlarge Image

×
Untreated, LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 30234-1-AP
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Untreated, LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IL-1 beta Recombinant antibodies (240902A3) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, ELISA, FC (Intra)

Conjugation

Unconjugated

CloneNo.

240902A3
-_- / -_-

Cat No. 98142-1-RR, 82696-11-PBS, 98142-1-PBS
MultiPro® 5CFLX Anti-Human IL-1 Beta (2A1B4)

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G66737-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

2A1B4

Sizes
-_- / -_-

Cat No. G66737-1-5C
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G66737-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G66737-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.
Rat IL-1 beta ELISA Kit

35 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

12.5 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE20005
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IL-1 beta Polyclonal antibody

6 Publications

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Untreated RAW 264.7 cells, RAW 264.7 cell (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for at least 3h) were subjected to SDS PAGE followed by western blot with 29530-1-AP (Il1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 29530-1-AP
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Untreated RAW 264.7 cells, RAW 264.7 cell (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for at least 3h) were subjected to SDS PAGE followed by western blot with 29530-1-AP (Il1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Rat IL-1 beta ELISA Kit

6 Publications

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

1.9 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE20021
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IL-1 beta E.Coli expressed Tagged-proteins (6 total products)

Species

human, mouse

Source

PET28a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag30589, Ag26030, Ag30574, Ag10295, Ag23465, Ag23461

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human IL-1 beta protein

-_- / -_-

IL-1 Beta Polyclonal antibody

-_- / -_-

IL-1 beta Recombinant antibodies (240902D6) (2 total products)

-_- / -_-

IL-1 Beta Monoclonal antibodies (2A1B4) (2 total products)

-_- / -_-

Mouse IL-1 beta ELISA Kit

-_- / -_-

IL-1 beta Polyclonal antibody

-_- / -_-

Human IL-1 beta ELISA Kit

-_- / -_-

IL-1 beta Recombinant antibodies (240902G6) (2 total products)

-_- / -_-

IL-1 beta Recombinant antibodies (241364D5) (2 total products)

-_- / -_-

IL-1 Beta Recombinant antibodies (3I9) (2 total products)

-_- / -_-

IL-1 beta Matched Antibody PairsNew

Anti-Human IL-1 beta (2A7C6) antibodies (6 total products)

-_- / -_-

HumanKine® recombinant human IL-1 beta protein- GMP grade

-_- / -_-

IL-1 Beta Polyclonal antibody

-_- / -_-

IL-1 beta Polyclonal antibody

-_- / -_-

IL-1 beta Recombinant antibodies (240902A3) (3 total products)

-_- / -_-

MultiPro® 5CFLX Anti-Human IL-1 Beta (2A1B4)

-_- / -_-

Rat IL-1 beta ELISA Kit

-_- / -_-

IL-1 beta Polyclonal antibody

-_- / -_-

Rat IL-1 beta ELISA Kit

-_- / -_-

IL-1 beta E.Coli expressed Tagged-proteins (6 total products)

-_- / -_-

Products derived from

IL-1 beta Matched Antibody Pairs^New