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52 product results for "IL-4"

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HumanKine® recombinant human IL-4 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

53 Publications

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Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.07-0.4 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1004
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Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.
HumanKine® recombinant human IL-4 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.07-0.4 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1004-GMP
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Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-4 (HZ-1004-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.07-0.4 ng/mL.
IL-4 Monoclonal antibodies (1G2C5) (3 total products)

KO/KD Validated

52 Publications

Host/IsoType

Mouse/IgG2a

Reactivity

human, mouse, rat, bovine, pig

Applications

ELISA, FC (Intra), IF/ICC, IF-P, IHC, WB

Conjugation

CoraLite®594, Unconjugated

CloneNo.

1G2C5
-_- / -_-

Cat No. 66142-1-Ig, CL594-66142, 66142-1-PBS
IL-4 Polyclonal antibody

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30533-1-AP (IL-4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

IHC, ELISA

Sizes
-_- / -_-

Cat No. 30533-1-AP
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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30533-1-AP (IL-4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30533-1-AP (IL-4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IL-4 Recombinant antibodies (240794E11) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

240794E11
-_- / -_-

Cat No. 98138-1-RR, 98138-1-PBS
Mouse IL-4 ELISA Kit

26 Publications

Reviews (1)

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE10010
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IL-4 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00829-3	83894-2-PBS	83894-5-PBS	IL-4	Human	Cytometric bead array, Sandwich ELISA	sELISA: 15.6-1000 pg/mL, CBA: 0.625-40 ng/mL
MP00829-1	83894-1-PBS	83894-4-PBS	IL-4	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00829-2	83894-3-PBS	83894-5-PBS	IL-4	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP50010-1	68728-1-PBS	68728-2-PBS	IL-4	Human	Sandwich ELISA	sELISA: 0.313-20 ng/mL
MP50049-1	68667-1-PBS	68728-2-PBS	IL-4	Human	Sandwich ELISA	sELISA: 15.6-500 pg/mL

Human IL-4 ELISA Kit

9 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

2.2 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE00016
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Anti-Mouse IL-4 (11B11) antibodies (8 total products)

1 Publications

Host/IsoType

Rat/IgG1

Reactivity

mouse

Applications

FC (Intra)

Conjugation

APC, CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, FITC Plus, PE, Unconjugated

CloneNo.

11B11
-_- / -_-

Cat No. 65074-1-Ig, PE-65074, CL488-65074, CL647-65074, APC-65074, CL555-65074, FITC-65074, CL750-65074
NeutraKine® IL-4 Monoclonal antibody

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Recombinant human IL-4 (Cat.NO. HZ-1004) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-4 (0.5 ng/mL HZ-1004) is neutralized by mouse anti-human IL-4 monoclonal antibody 69005-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 200-500 ng/mL.

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Neutralization

CloneNo.

1H5G5

Sizes
-_- / -_-

Cat No. 69005-1-Ig
×

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Recombinant human IL-4 (Cat.NO. HZ-1004) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-4 (0.5 ng/mL HZ-1004) is neutralized by mouse anti-human IL-4 monoclonal antibody 69005-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 200-500 ng/mL.
IL-4 Recombinant antibodies (240794A8) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, ELISA, IF/ICC

Conjugation

Unconjugated

CloneNo.

240794A8
-_- / -_-

Cat No. 83894-1-RR, 83894-1-PBS

Il4 Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP01293-4	84422-3-PBS	84422-5-PBS	Il4	Rat	Sandwich ELISA	sELISA: 39.1-2500 pg/mL
MP01293-1	84422-4-PBS	84422-1-PBS	Il4	Rat	Cytometric bead array	CBA: 0.625-80 ng/mL
MP01293-2	84422-3-PBS	84422-2-PBS	Il4	Rat	Cytometric bead array	CBA: 0.625-80 ng/mL
MP01293-3	84422-5-PBS	84422-2-PBS	Il4	Rat	Cytometric bead array	CBA: 1.25-80 ng/mL

Recombinant human IL-4 protein

1 Publications

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Species

human

Source

E. coli-derived, PET28a

Tag

6*His

Format

Powder

Sizes
-_- / -_-

Cat No. Ag9940
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IL-4 fusion protein Ag9940 SDS-PAGE
STAT6 Recombinant antibodies (4F19) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

4F19
-_- / -_-

Cat No. CL488-82630, 82630-1-PBS, 82630-1-RR
STAT6 Polyclonal antibody

KO/KD Validated

26 Publications

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WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, RIP, ELISA

Sizes
-_- / -_-

Cat No. 51073-1-AP
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WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
STAT6 Monoclonal antibodies (2E5B8) (2 total products)

4 Publications

Host/IsoType

Mouse/IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

2E5B8
-_- / -_-

Cat No. 66717-1-Ig, 66717-1-PBS
IHCeasy STAT6 Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using KHC1151 (STAT6 IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human, mouse

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
-_- / -_-

Cat No. KHC1151
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Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC1151 (STAT6 IHC Kit).
STAT6 E.Coli expressed Tagged-proteins (2 total products)

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag0410, Ag23607