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96 product results for "IL-6"

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HumanKine® recombinant human IL-6 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

45 Publications

Reviews (1)

View More Images (3)

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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.03-0.24 ng/mL in 3G12B10 cells.

Expression

HEK293

Reactivity

human, mouse, rat, monkey, rabbit

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1019
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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL. 

Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL.
HumanKine® recombinant human IL-6 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

View More Images (2)

×
Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.03-0.24 ng/mL

Expression

HEK293

Reactivity

human, monkey

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1019-GMP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-6 (HZ-1019-GMP) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of GMP recombinant IL-6 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.03-0.24 ng/mL.
IL-6 Recombinant antibodies (240670F10) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

240670F10
-_- / -_-

Cat No. 83747-5-PBS, 83747-5-RR
IL-6 Polyclonal antibody

KO/KD Validated

628 Publications

Reviews (2)

View More Images (3)

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Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, canine, goat, pig, rabbit

Applications

WB, IHC, IP, Neutralization, ELISA

Sizes
-_- / -_-

Cat No. 21865-1-AP
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Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-IL-6 (IP:21865-1-AP, 4ug; Detection:21865-1-AP 1:1000) with LPS and Protein transport inhibitor treated HUVEC cells lysate 800 ug.

Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (21865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IL-6 Monoclonal antibodies (1A3B4) (2 total products)

KO/KD Validated

199 Publications

Host/IsoType

Mouse/IgG2b

Reactivity

human, pig, sheep

Applications

IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

1A3B4
-_- / -_-

Cat No. 66146-1-Ig, 66146-1-PBS

IL-6 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50032-1	68659-1-PBS	68659-2-PBS	IL-6	human	Cytometric bead array, Sandwich ELISA	sELISA: 7.8-500 pg/mL, CBA: 0.098-12.5 ng/mL
MP00264-1	83179-1-PBS	83179-3-PBS	IL-6	Rat	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00264-2	83179-2-PBS	83179-3-PBS	IL-6	Rat	Cytometric bead array, Sandwich ELISA	sELISA: 62.5-4000 pg/mL, CBA: 0.625-20 ng/mL
MP00264-3	83179-2-PBS	83179-1-PBS	IL-6	Rat	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00687-1	83747-2-PBS	83747-4-PBS	IL-6	Human	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00687-2	83747-2-PBS	83747-1-PBS	IL-6	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00687-3	83747-2-PBS	83747-3-PBS	IL-6	Human	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00687-4	83747-2-PBS	83747-6-PBS	IL-6	Human	Sandwich ELISA	sELISA: 7.8-500 pg/mL
MP00756-1	83795-1-PBS	83795-3-PBS	IL-6	Mouse	Cytometric bead array	CBA: 1.25-40 ng/mL
MP00756-2	83795-4-PBS	83795-2-PBS	IL-6	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00756-3	83795-5-PBS	83795-2-PBS	IL-6	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00756-4	83795-6-PBS	83795-1-PBS	IL-6	Mouse	Sandwich ELISA	sELISA: 15.6-1000 pg/mL
MP50032-2	68659-3-PBS	66146-2-PBS	IL-6	Human	Cytometric bead array	CBA: 1.563-100 ng/mL
MP50032-3	68659-13-PBS	68659-10-PBS	IL-6	Human	Cytometric bead array	CBA: 3.125-50 ng/mL
MP80002-1	81077-1-PBS	68659-10-PBS	IL-6	Human	Cytometric bead array	CBA: 1.563-100 ng/mL