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39 product results for "IL-8"

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HumanKine® recombinant human IL-8 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

3 Publications

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Purity of recombinant human IL-8 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

100-500 ng/mL

Expression

HEK293

Reactivity

human

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1318
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Purity of recombinant human IL-8 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IL-8 (HZ-1318) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL. 

Proteintech IL-8 (HZ-1318) demonstrates a 2.5-fold decrease in EC50 and a greater fold induction compared to leading competitors. Recombinant human IL-8 stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.
CXCL8/IL-8 Recombinant antibodies (240889C4) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB

Conjugation

Unconjugated

CloneNo.

240889C4
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Cat No. 83903-4-RR, 83903-4-PBS
CXCL8/IL-8 Polyclonal antibody

46 Publications

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PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27095-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, pig, zebrafish

Applications

WB, IHC, IF, ELISA, Cell treatment

Sizes
-_- / -_-

Cat No. 27095-1-AP
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PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27095-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27095-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27095-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Human CXCL8/IL-8 ELISA Kit

61 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1.0 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE00006
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CXCL8/IL-8 Polyclonal antibodies (2 total products)

21 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine

Applications

IF/ICC, IHC, Single Cell, WB

Conjugation

5CFLX, Unconjugated
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Cat No. 17038-1-AP, G17038-1-5C

CXCL8/IL-8 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00830-3	83903-5-PBS	83903-1-PBS	CXCL8/IL-8	Human	Sandwich ELISA	sELISA: 2.73-175 pg/mL
MP00830-1	83903-3-PBS	83903-1-PBS	CXCL8/IL-8	Human	Cytometric bead array	CBA: 0.625-40 ng/mL
MP00830-2	83903-2-PBS	83903-1-PBS	CXCL8/IL-8	Human	Cytometric bead array	CBA: 0.625-40 ng/mL
MP50089-1	68744-1-PBS	60141-2-PBS	CXCL8/IL-8	human	Sandwich ELISA	sELISA: 0.049-3.125 ng/mL

AuthentiKine™ Human CXCL8/IL-8 ELISA Kit

3 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

0.2 pg/mL

Range

1.56-100 pg/mL

Sizes
-_- / -_-

Cat No. KE00275
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CXCL8,IL8 Recombinant antibodies (240889B2) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

240889B2
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Cat No. 98137-1-PBS, 98137-1-RR
CXCL8/IL-8 Polyclonal antibody

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Untreated, PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 31533-1-AP
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Untreated, PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31533-1-AP (CXCL8/IL-8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0801 (CXCL8/IL-8 IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
-_- / -_-

Cat No. KHC0801
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0801 (CXCL8/IL-8 IHC Kit).
IL-8 E.Coli expressed Tagged-proteins (3 total products)

1 Publications

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag17729, Ag25640, Ag26227
CXCR1 Recombinant antibodies (240819A11) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

240819A11
-_- / -_-

Cat No. 83801-4-RR, 83801-4-PBS

CXCR1 Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00731-3	83801-6-PBS	83801-5-PBS	CXCR1	Human	Sandwich ELISA	sELISA: 0.781-50 ng/mL
MP00731-1	83801-2-PBS	83801-1-PBS	CXCR1	Human	Cytometric bead array	CBA: 1.25-80 ng/mL
MP00731-2	83801-2-PBS	83801-3-PBS	CXCR1	Human	Cytometric bead array	CBA: 5-80 ng/mL

CXCR2 Polyclonal antibodies (2 total products)

KO/KD Validated

15 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

CoIP, IF/ICC, IF-P, IHC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
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Cat No. CL488-20634, 20634-1-AP
CXCR1 Polyclonal antibody

KO/KD Validated

8 Publications

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Jurkat cells were subjected to SDS PAGE followed by western blot with 55450-1-AP (CXCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IF, IP, ELISA

Sizes
-_- / -_-

Cat No. 55450-1-AP
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Jurkat cells were subjected to SDS PAGE followed by western blot with 55450-1-AP (CXCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IP result of anti-CXCR1 (IP:55450-1-AP, 4ug; Detection:55450-1-AP 1:500) with Jurkat cells lysate 4000ug.
IL-8RB/CXCR2 Fusion Protein

Enlarge Image

Species

human

Source

E. coli-derived, PGEX-4T

Tag

GST

Format

Powder

Sizes
-_- / -_-

Cat No. Ag14670
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IL-8RB/CXCR2 fusion protein Ag14670 SDS-PAGE

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human IL-8 protein

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CXCL8/IL-8 Recombinant antibodies (240889C4) (2 total products)

-_- / -_-

CXCL8/IL-8 Polyclonal antibody

-_- / -_-

Human CXCL8/IL-8 ELISA Kit

-_- / -_-

CXCL8/IL-8 Polyclonal antibodies (2 total products)

-_- / -_-

CXCL8/IL-8 Matched Antibody PairsNew

AuthentiKine™ Human CXCL8/IL-8 ELISA Kit

-_- / -_-

CXCL8,IL8 Recombinant antibodies (240889B2) (2 total products)

-_- / -_-

CXCL8/IL-8 Polyclonal antibody

-_- / -_-

IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit

-_- / -_-

IL-8 E.Coli expressed Tagged-proteins (3 total products)

-_- / -_-

CXCR1 Recombinant antibodies (240819A11) (2 total products)

-_- / -_-

CXCR1 Matched Antibody PairsNew

CXCR2 Polyclonal antibodies (2 total products)

-_- / -_-

CXCR1 Polyclonal antibody

-_- / -_-

IL-8RB/CXCR2 Fusion Protein

-_- / -_-

Products derived from

CXCL8/IL-8 Matched Antibody Pairs^New

CXCR1 Matched Antibody Pairs^New