1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 594 Anti-Mouse IgG2a Isotype Control (CL594-65244, Clone:MOPC-173) (red), unstained (blue), or CL594 Anti-Human CD3 (Hit3a) (green). Cells were not fixed. Lymphocytes were gated.

BLI binding kinetics of a mouse IgG1 anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal mouse IgG1 anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

BLI binding kinetics of a monoclonal rabbit IgG anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal rabbit IgG anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

BLI binding kinetics of a purified and a hybridoma supernatant mouse IgG1 anti-Halo antibody to Halo-tag. A purified, monoclonal mouse IgG1 anti-Halo antibody (blue, ChromoTek Halo antibody [28A8]) and a hybridoma supernatant, monoclonal mouse IgG1 anti-Halo antibody (green) were immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of Halo-tag protein.

ELISA capture of mouse IgG1 antibody using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Mouse IgG1 antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

Species reactivity of Secondary VHH.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-B23 (60096-1-lg) labeled with FlexAble CoraLite® Plus 594 Kit (KFA029, orange) and DAPI (blue). ​ Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA029). Cells are not fixed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA029) and co-stained anti-CD4 (CL750-65134) antibody. Cells are not fixed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD45RA (clone F8-11-13, 65226-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA029) and co-stained anti-CD45RO (CL750-65150) antibody. Cells are not fixed.

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 647 Anti-Human CD45RO (CL647-65150, Clone:UCHL1), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 647 Anti-Human CD3 (CL647-65151, Clone:UCHT1), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 647 Anti-Human CD45 (CL647-65109, Clone:HI30), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD20 (65085-1-Ig, Clone:2H7) labeled with FlexAble CoraLite® Plus 647 Kit (KFA563), and Anti-Human CD45 (65109-1-Ig, MsIgG1 Clone:HI30) labeled with FlexAble CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD20 (65085-1-Ig, Clone:2H7) labeled with FlexAble CoraLite® Plus 647 Kit (KFA563), and Anti-Human CD45 (65109-1-Ig, MsIgG1 Clone:HI30) labeled with FlexAble CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, orange), anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, orange), anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, orange) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, orange) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with Multi-rAb CoraLite® Plus 488 Goat anti-Mouse Recombinant Secondary (RGAM002, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, red) and mouse IgG1 anti-ATP5O (66696-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with Multi-rAb CoraLite® Plus 488 Goat anti-Mouse Recombinant Secondary (RGAM002, green), mouse IgG1 anti-Lamin antibody (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, red) and mouse IgG1 anti-ATP5O antibody (66696-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-Lamin antibody (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, red). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 647 Anti-Human CD45RO (CL647-65150, Clone:UCHL1), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 647 Anti-Human CD3 (CL647-65151, Clone:UCHT1), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 647 Anti-Human CD45 (CL647-65109, Clone:HI30), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522). Cells were not fixed. Lymphocytes were gated.

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta),anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, orange) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta),anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, orange) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite® Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite® Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite® Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite® Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with Multi-rAb CoraLite® Plus 488 Goat anti-Mouse Recombinant Secondary (RGAM002, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, red) and mouse IgG1 anti-ATP5O (66696-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with Multi-rAb CoraLite® Plus 488 Goat anti-Mouse Recombinant Secondary (RGAM002, green), mouse IgG1 anti-Lamin antibody (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, red) and mouse IgG1 anti-ATP5O antibody (66696-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta).Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 488 Anti-Human CD3 (CL488-65151, Clone:UCHT1), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 488 Anti-Human CD45 (CL488-65109, Clone:HI30), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523). Cells were not fixed. Lymphocytes were gated.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite® Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite® Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite® Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite® Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody (66041-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 488 Anti-Human CD3 (CL488-65151, Clone:UCHT1), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 488 Anti-Human CD45 (CL488-65109, Clone:HI30), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD4 antibody (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

FlexAble Workflow

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, blue), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, yellow) and anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, grey).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Ki67 labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green).

FlexAble Workflow

Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN (66836-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), anti-TUBB3 (66375-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

WB of HEK293 cell lysates: siRNA transfected HEK293 cell lysates were detected with anti-GNA13 (67188-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022,green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

FlexAble Workflow

Immunofluorescence of human kidney: FFPE human kidney sections were stained with anti-Calbindin (14479-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-ACE2 (66699-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), CoraLite®488-conjugated Podocalyxin antibody (CL488-18150, green) and DAPI (blue).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, yellow), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan) and DAPI (blue). ​Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Vimentin labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, yellow) and anti-hnRNP labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024).

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) and anti-CD8 (clone RPA-T8, 65144-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

WB of A431 cell lysate: siRNA transfected A431 cell lysates were detected with anti-P53 (80077-1-RR) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, cyan) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024).

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, red).

FlexAble Workflow

Various cell lysates were subject to SDS PAGE followed by western blot with anti-HSPA5 antibody (66574-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Vimentin antibody (60330-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Beta Catenin antibody (66379-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-GORASP2 antibody (66627-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

WB of HeLa and HEK-293 Cell lysates (30 μg/lane): Various cell lysates were detected with anti-Beta Catenin 66379-1-Ig, Mouse IgG1, 0.44 μg) labeled with FlexAble HRP Kit (KFA025, 0.88 μL) (Lane 1 and Lane 2); Anti- GORASP2 (66627-1-Ig, Mouse IgG1, 0.18 μg) with FlexAble HRP Kit (KFA025, 0.36 μL) (Lane 3 and Lane 4); anti-Beta Catenin (66379-1-Ig, Mouse IgG1, 0.44 μg) labeled with FlexAble HRP Kit (KFA025, Linker 1.76 μL) with Quencher 0.88 μL, add 0.18 μg GORASP2 antibody as competitor antibody (Lane 5 and Lane 6). Primary antibody : Linker : Quencher=0.5 μg : 1 μL : 2 μL

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025). Antibody used at dilution of 1:400 under 10x & 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025). Antibody used at dilution of 1:400 under 10x & 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-ATP5O antibody (66696-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-594 (red).  Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.