1x10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-13 Rabbit Recombinant Antibody, PBS Only (98003-1-PBS, Clone:2C14) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
