Results

118 product results for "MAPK"

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ERK-MAPK Pathway Antibody Kit

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Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Host

Rabbit/Mouse

Reactivity

Target Dependent

Applications

Target Dependent

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. PK30019
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Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated A431 and Nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

UV treated and non-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80086-1-RR (Phospho-JUN (Ser73) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control.

Non-treated HEK-293T cells, phosphatase inhibitor and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81398-1-RR (Phospho-MNK1 (Thr250/255) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells untreated (dashed lines) or Calyculin A (red) treated were intracellularly stained with 0.13 ug Anti-Human Phospho-MEK1 (Thr292) (67873-1-Ig, Clone:2D7A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
ERK1/2 Polyclonal antibodies (4 total products)

KO/KD Validated

355 Publications

Reviews (2)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, canine, chicken, pig, sheep

Applications

CoIP, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, Unconjugated
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Cat No. CL488-11257, CL647-11257, 11257-1-AP, CL555-11257
Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

KO/KD Validated

370 Publications

Reviews (3)

View More Images (3)

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Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (ERK1/2-phospho-Thr202/Tyr204) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, pig, sheep

Applications

WB, IHC, IF, IP, ELISA

Sizes
-_- / -_-

Cat No. 28733-1-AP
×

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Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (ERK1/2-phospho-Thr202/Tyr204) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

IP result of anti-Phospho-ERK1/2 (Thr202/Tyr204) (IP:28733-1-AP, 2ug; Detection:28733-1-AP 1:1000) with Calyculin A treated PC-3 cells lysate 1552 ug.

Non-treated and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibodies (8D12) (2 total products)

26 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

8D12
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Cat No. 80031-1-PBS, 80031-1-RR
ERK1/2 Recombinant antibodies (4P8) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

4P8
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Cat No. CL488-83533, 83533-1-RR, 83533-1-PBS

ERK1 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50383-1	67170-2-PBS	66192-2-PBS	ERK1	Human	Cytometric bead array	CBA: 3.125-50 ng/mL
MP50383-2	66192-3-PBS	66192-2-PBS	ERK1	Human	Cytometric bead array	CBA: 1.563-25 ng/mL

ERK1/2 Monoclonal antibodies (4G8A11) (3 total products)

33 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat

Applications

CoIP, ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

4G8A11
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Cat No. CL488-66192, 66192-1-Ig, 66192-1-PBS
ERK1/2 Fusion Protein

Enlarge Image

Species

human

Source

E. coli-derived, PGEX-4T

Tag

GST

Format

Powder

Sizes
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Cat No. Ag1759
×

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ERK1/2 fusion protein Ag1759 SDS-PAGE
p38 MAPK Polyclonal antibody

KO/KD Validated

415 Publications

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WB result of p38 antibody (14064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 transfected Jurkat cells.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, duck, fish, goat, pig, rabbit, zebrafish

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 14064-1-AP
×

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More validation images available on the product page

WB result of p38 antibody (14064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 transfected Jurkat cells.

Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Phospho-JNK (Tyr185) Recombinant antibodies (2G8) (2 total products)

202 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, pig, sheep

Applications

ELISA, IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

2G8
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Cat No. 80024-1-PBS, 80024-1-RR
Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibody

163 Publications

View More Images (5)

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Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 28796-1-AP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
JNK Polyclonal antibodies (2 total products)

158 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, pig

Applications

CoIP, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
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Cat No. 24164-1-AP, CL488-24164
JNK Monoclonal antibodies (1A12E1) (4 total products)

KO/KD Validated

147 Publications

Reviews (1)

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat, pig, rabbit, sheep

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

1A12E1
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Cat No. 66210-1-Ig, 66210-1-PBS, CL488-66210, CL594-66210
JNK Recombinant antibodies (6J16) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

6J16
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Cat No. 81629-1-RR, 81629-1-PBS, CL488-81629
p38 MAPK Recombinant antibodies (230263D11) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, FC (Intra), IF/ICC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

230263D11
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Cat No. 80821-2-RR, CL488-80821-2, 80821-2-PBS
p38 MAPK Recombinant antibodies (230263H7) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

ELISA, FC (Intra), IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

230263H7
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Cat No. 80821-3-PBS, CL488-80821-3, 80821-3-RR
JNK Polyclonal antibody

123 Publications

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4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, pig

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Sizes
-_- / -_-

Cat No. 51151-1-AP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Phospho-MNK1 (Thr250/255) Recombinant antibodies (4P9) (2 total products)

KO/KD Validated

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

4P9
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Cat No. 81398-1-RR, 81398-1-PBS
p38 MAPK Monoclonal antibodies (1A1C2) (2 total products)

KO/KD Validated

71 Publications

Reviews (1)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, pig

Applications

Cytometric bead array, ELISA, IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

1A1C2
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Cat No. 66234-1-PBS, 66234-1-Ig
ERK1/2 Polyclonal antibodies (3 total products)

35 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 647, CoraLite®555, Unconjugated
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Cat No. CL555-51068, CL647-51068, 51068-1-AP
JNK Polyclonal antibody

29 Publications

View More Images (4)

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NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 10023-1-AP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (10023-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).