Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.
WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Occludin antibody (66378-1-Ig, Clone: 1D3C4 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.