Various lysates were subjected to SDS PAGE followed by western blot with 60300-1-Ig (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTEN antibody (60300-1-Ig, Clone: 5C10B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).