Various lysates were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
A549 cells were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX2/ Cyclooxygenase 2/ PTGS2 antibody (12375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using COX2/ Cyclooxygenase 2/ PTGS2 antibody (12375-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).