Various lysates were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.
WB result of SOD2 antibody (66474-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD2 transfected HeLa cells. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (66474-1-Ig, Clone: 3A6C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.