Results

37 product results for "TGFb"

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HumanKine® recombinant human TGF beta 1 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

193 Publications

Reviews (3)

View More Images (3)

×
Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Activity

Typically ≤ 0.5 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat, goat, pig

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1011
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Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL. 

Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)
HumanKine® recombinant human TGF beta 1 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

1 Publications

View More Images (2)

×
Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Activity

0.01-0.17 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1011-GMP
×

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Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.
TGF Beta 1 Recombinant antibodies (230544B7) (3 total products)

1 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 750, Unconjugated

CloneNo.

230544B7
-_- / -_-

Cat No. 81746-2-PBS, 81746-2-RR, CL750-81746-2
NeutraKine® TGF beta 1 Monoclonal antibody

6 Publications

View More Images (4)

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Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

IHC, Neutralization, ELISA

CloneNo.

1E3E9

Sizes
-_- / -_-

Cat No. 69012-1-Ig
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Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
TGF beta 1 Polyclonal antibody

2 Publications

View More Images (3)

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Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Sizes
-_- / -_-

Cat No. 26155-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TGF beta 1 antibody (26155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug TGF beta 1 Polyclonal antibody (26155-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Human TGF-beta1 ELISA Kit

51 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants, Urine

Sensitivity

3.3 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE00002
×

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Mouse TGF-beta1 ELISA Kit

47 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

16.0 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE10005
×

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Rat TGF-beta1 ELISA Kit

10 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

3.4 pg/mL

Range

31.25 - 2000 pg/mL

Sizes
-_- / -_-

Cat No. KE20010
×

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IHCeasy TGF beta 1 Ready-To-Use IHC Kit

1 Publications

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×
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0131 (TGF beta 1 IHC Kit).

Primary Ab Type

Mouse Monoclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC0131
×

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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0131 (TGF beta 1 IHC Kit).
NeutraControl TGF beta 1 Monoclonal antibody

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×
Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Non-Neutralization

CloneNo.

1D3D11

Sizes
-_- / -_-

Cat No. 69512-1-Ig
×

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Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
TGF Beta 1 Polyclonal antibody

KO/KD Validated

683 Publications

Reviews (8)

View More Images (3)

×
Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, canine, duck, pig, rabbit, zebrafish

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Sizes
-_- / -_-

Cat No. 21898-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
TGFB1 E.Coli expressed Tagged-proteins (3 total products)

Species

human

Source

PET28a, PET30a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag24881, Ag27942, Ag24877
LTBP1 Polyclonal antibody

KO/KD Validated

5 Publications

Reviews (1)

View More Images (4)

×
human plasma were subjected to SDS PAGE followed by western blot with 26855-1-AP (LTBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 26855-1-AP
×

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human plasma were subjected to SDS PAGE followed by western blot with 26855-1-AP (LTBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26855-1-AP (LTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
LTBP1 Polyclonal antibody

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mouse liver tissue were subjected to SDS PAGE followed by western blot with 22065-1-AP (LTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 22065-1-AP
×

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mouse liver tissue were subjected to SDS PAGE followed by western blot with 22065-1-AP (LTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22065-1-AP (LTBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22065-1-AP (LTBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
LTBP1 E.Coli expressed Tagged-proteins (2 total products)

Species

human

Source

PET30a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag25392, Ag17141
HumanKine® recombinant human TGF beta 2 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

8 Publications

View More Images (2)

×
Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.018-0.18 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1092
×

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Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human TGF beta 2 (HZ-1092) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.018-0.18 ng/mL.
HumanKine® recombinant human TGF beta 2 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

View More Images (2)

×
Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.018-0.18 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1092-GMP
×

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More validation images available on the product page

Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human TGF beta 2 (HZ-1092-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.018-0.18 ng/mL.
TGF beta 2-Specific Polyclonal antibody

KO/KD Validated

49 Publications

Reviews (1)

View More Images (4)

×
Various lysates were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Sizes
-_- / -_-

Cat No. 19999-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19999-1-AP (TGF-beta 2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
TGFB2/TGF-beta 2 Polyclonal antibody

7 Publications

View More Images (4)

×
Various lysates were subjected to SDS PAGE followed by western blot with 28426-1-AP (TGF beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 28426-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 28426-1-AP (TGF beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28426-1-AP (TGF beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28426-1-AP (TGF beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TGF beta 2 antibody (28426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
TGIF2 Polyclonal antibody

KO/KD Validated

5 Publications

View More Images (3)

×
Various lysates were subjected to SDS PAGE followed by western blot with 11522-1-AP (TGIF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 11522-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 11522-1-AP (TGIF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using TGIF2 antibody (11522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
TGIF2 Monoclonal antibodies (2D6B6) (2 total products)

1 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, FC (Intra), WB

Conjugation

Unconjugated

CloneNo.

2D6B6
-_- / -_-

Cat No. 67576-1-PBS, 67576-1-Ig

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human TGF beta 1 protein

-_- / -_-

HumanKine® recombinant human TGF beta 1 protein- GMP grade

-_- / -_-

TGF Beta 1 Recombinant antibodies (230544B7) (3 total products)

-_- / -_-

NeutraKine® TGF beta 1 Monoclonal antibody

-_- / -_-

TGF beta 1 Polyclonal antibody

-_- / -_-

Human TGF-beta1 ELISA Kit

-_- / -_-

Mouse TGF-beta1 ELISA Kit

-_- / -_-

Rat TGF-beta1 ELISA Kit

-_- / -_-

IHCeasy TGF beta 1 Ready-To-Use IHC Kit

-_- / -_-

NeutraControl TGF beta 1 Monoclonal antibody

-_- / -_-

TGF Beta 1 Polyclonal antibody

-_- / -_-

TGFB1 E.Coli expressed Tagged-proteins (3 total products)

-_- / -_-

LTBP1 Polyclonal antibody

-_- / -_-

LTBP1 Polyclonal antibody

-_- / -_-

LTBP1 E.Coli expressed Tagged-proteins (2 total products)

-_- / -_-

HumanKine® recombinant human TGF beta 2 protein

-_- / -_-

HumanKine® recombinant human TGF beta 2 protein- GMP grade

-_- / -_-

TGF beta 2-Specific Polyclonal antibody

-_- / -_-

TGFB2/TGF-beta 2 Polyclonal antibody

-_- / -_-

TGIF2 Polyclonal antibody

-_- / -_-

TGIF2 Monoclonal antibodies (2D6B6) (2 total products)

-_- / -_-

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