Results for "TNFa" | Proteintech Group

50 product results for "TNFa"

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TNF-alpha Recombinant antibodies (240702B7) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

240702B7
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Cat No. 80258-6-PBS, 80258-6-RR
TNF-alpha Polyclonal antibody

3 Publications

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Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26162-1-AP (Tnf alpha antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 26162-1-AP
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Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26162-1-AP (Tnf alpha antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.
Mouse TNF-alpha ELISA Kit

330 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE10002
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TNF alpha Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00682-1	80258-3-PBS	80258-2-PBS	TNF-alpha	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00682-2	80258-4-PBS	80258-2-PBS	TNF-alpha	Mouse	Cytometric bead array	CBA: 0.156-20 ng/mL
MP00682-3	80258-5-PBS	80258-2-PBS	TNF-alpha	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00702-1	83721-3-PBS	83721-2-PBS	TNF-alpha	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00702-2	83721-5-PBS	83721-4-PBS	TNF-alpha	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00702-3	83721-1-PBS	83721-2-PBS	TNF-alpha	Human	Cytometric bead array	CBA: 0.628-40 ng/mL
MP00702-4	83721-5-PBS	83721-6-PBS	TNF-alpha	Human	Sandwich ELISA	sELISA: 3.9-250 pg/mL
MP50093-1	68740-1-PBS	68740-2-PBS	TNF alpha	Human	Sandwich ELISA	sELISA: 0.020-5 ng/mL

HumanKine® recombinant human TNF alpha protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

52 Publications

Reviews (2)

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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.002-0.026 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1014
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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL. 

Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.
HumanKine® recombinant human TNF alpha protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.002-0.026 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1014-GMP
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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human TNF alpha (HZ-1014-GMP) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.002-0.026 ng/mL.
TNF-alpha Monoclonal antibodies (7B8A11) (4 total products)

KO/KD Validated

469 Publications

Reviews (2)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, bovine, chicken, duck, monkey, pig, rabbit

Applications

ELISA, IF-P, IHC, RIP, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

7B8A11
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Cat No. CL488-60291, 60291-1-Ig, 60291-1-PBS, CL594-60291
TNF-alpha Polyclonal antibody

669 Publications

Reviews (2)

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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, fish, rabbit, sheep

Applications

WB, ELISA, Cell treatment, Transwell Assay

Sizes
-_- / -_-

Cat No. 17590-1-AP
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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
TNF-alpha Recombinant antibodies (240668B4) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB

Conjugation

Unconjugated

CloneNo.

240668B4
-_- / -_-

Cat No. 83721-7-PBS, 83721-7-RR
AuthentiKine™ Human TNF-alpha ELISA Kit

58 Publications

Reviews (2)

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

8.6 pg/mL

Range

15.6-500 pg/mL

Sizes
-_- / -_-

Cat No. KE00154
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Rat TNF-alpha ELISA Kit

57 Publications

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Assay Type

Sandwich

Sample Type

Cell culture supernatants

Sensitivity

7.7 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE20001
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TNF-alpha Polyclonal antibody

30 Publications

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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 26405-1-AP (TNF alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 26405-1-AP
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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 26405-1-AP (TNF alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 26405-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody 60004-1-Ig as loading control.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26405-1-AP (TNF Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26405-1-AP (TNF Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26405-1-AP (TNF Alpha antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Rat TNF-alpha ELISA Kit

7 Publications

Reviews (1)

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

1.1 pg/mL

Range

4.7-300 pg/mL

Sizes
-_- / -_-

Cat No. KE20018
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NeutraKine® TNF-alpha Monoclonal antibodies (11H8A5) (4 total products)

2 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse

Applications

FC (Intra), Neutralization & Neutro-controls

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, Unconjugated

CloneNo.

11H8A5
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Cat No. CL647-69002, CL488-69002, 69002-1-PBS, 69002-1-Ig
TNF-alpha Polyclonal antibody

1 Publications

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PMA and ionomycin treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 29652-1-AP (TNF-alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 29652-1-AP
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PMA and ionomycin treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 29652-1-AP (TNF-alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Human TNF-alpha ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

0.4 pg/mL

Range

3.9-250 pg/mL

Sizes
-_- / -_-

Cat No. KE00367
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Standard Curve of KE00367 Human TNF-alpha ELISA Kit
IHCeasy TNF Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using KHC1155 (TNF IHC Kit).

Primary Ab Type

Mouse Monoclonal

Reactivity

human, mouse

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC1155
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Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using KHC1155 (TNF IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using KHC1155 (TNF IHC Kit).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using KHC1155 (TNF IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using KHC1155 (TNF IHC Kit).
MultiPro® 5CFLX Anti-Human TNF Alpha (7B8A11)

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G60291-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Host/IsoType

Mouse/IgG2b

Reactivity

human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

7B8A11

Sizes
-_- / -_-

Cat No. G60291-1-5C
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G60291-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G60291-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.
NeutraControl TNF-alpha Monoclonal antibody

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Recombinant human TNF alpha (Cat.NO. HZ-1014) inhibits the growth of L-929 cell line in the presence of actinomycin D in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity elicited by recombinant human TNF alpha (0.05 ng/mL HZ-1014) is neutralized by mouse anti-human TNF alpha monoclonal antibody 69002-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 10-50 ng/mL. The NeutraControl mouse anti-human TNF alpha monoclonal antibody 69502-1-Ig, generated from the same immunogen, could recognize human TNF alpha, but could not neutralize human TNF alpha at the range of 0.128-10000ng/mL (green curve, refer to top X-right Y).

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse

Applications

ELISA, Non-Neutralization

CloneNo.

1A3A3

Sizes
-_- / -_-

Cat No. 69502-1-Ig
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Recombinant human TNF alpha (Cat.NO. HZ-1014) inhibits the growth of L-929 cell line in the presence of actinomycin D in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity elicited by recombinant human TNF alpha (0.05 ng/mL HZ-1014) is neutralized by mouse anti-human TNF alpha monoclonal antibody 69002-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 10-50 ng/mL. The NeutraControl mouse anti-human TNF alpha monoclonal antibody 69502-1-Ig, generated from the same immunogen, could recognize human TNF alpha, but could not neutralize human TNF alpha at the range of 0.128-10000ng/mL (green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TNF Alpha (Cat.NO. HZ-1014) at 70 ng/well followed by blocking and adding serial diluted TNF Alpha antibody 69002-1-Ig and 69502-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
TNF-alpha Recombinant antibodies (240668B11) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

240668B11
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Cat No. 98064-1-RR, 98064-1-PBS
TNF-alpha E.Coli expressed Tagged-proteins (2 total products)

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag11413, Ag24020