HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of Hela cells, using PIK3R4 antibody 17894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).