PC-3 cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB result of VPS34 (C terminal) antibody (12452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 (C terminal) transfected Jurkat cells.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using VPS34 (C terminal) antibody (12452-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
HeLa cells were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB result of VPS34 antibody (13723-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 transfected Jurkat cells.
Immunohistochemical analysis of paraffin-embedded human heart using 13723-1-AP (VPS34 antibody) at dilution of 1:100 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human heart using 13723-1-AP (VPS34 antibody) at dilution of 1:100 (under 40x lens).
Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29675-1-AP (Phospho-VPS34 (Thr159) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.
