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29 product results for "Vimentin"

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Vimentin Monoclonal antibodies (3H9D1) (6 total products)

KO/KD Validated

258 Publications

Reviews (3)

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat, zebrafish

Applications

CoIP, Cytometric bead array, ELISA, IF/ICC, IF-P, IHC, IP, WB

Conjugation

Biotin, CoraLite®488, CoraLite®555, CoraLite®594, Unconjugated

CloneNo.

3H9D1
-_- / -_-

Cat No. CL488-60330, 60330-1-Ig, CL594-60330, 60330-1-PBS, CL555-60330, Biotin-60330
Vimentin Recombinant antibodies (6K21) (6 total products)

KO/KD Validated

2 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®555, CoraLite®594, Unconjugated

CloneNo.

6K21
-_- / -_-

Cat No. CL488-80232, CL555-80232, CL594-80232, 80232-1-RR, 80232-1-PBS, CL647-80232
Vimentin Polyclonal antibodies (4 total products)

1649 Publications

Reviews (10)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, deer, goat, hamster, pig, sheep

Applications

CoIP, FC (Intra), IF/ICC, IF-Fro, IF-P, IHC, IP, RIP, Single Cell, WB

Conjugation

5CFLX, CoraLite® Plus 488, CoraLite®594, Unconjugated
-_- / -_-

Cat No. G10366-1-5C, CL594-10366, 10366-1-AP, CL488-10366

VIM Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50158-1	60330-2-PBS	60330-1-PBS	VIM	Human	Cytometric bead array	CBA: 3.125-100 ng/mL

IHCeasy Vimentin Ready-To-Use IHC Kit

2 Publications

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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0039 (Vimentin IHC Kit).

Primary Ab Type

Mouse Monoclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC0039
×

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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0039 (Vimentin IHC Kit).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC0039 (Vimentin IHC Kit).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0039 (Vimentin IHC Kit).

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using KHC0039 (Vimentin IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0039 (Vimentin IHC Kit).
MultiPro® 5CFLX Anti-Human Vimentin (3H9D1)

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G60330-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

3H9D1

Sizes
-_- / -_-

Cat No. G60330-1-5C
×

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More validation images available on the product page

G60330-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G60330-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.
Vimentin-Label Atto488

2 Publications

Enlarge Image

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MDCK cells were immunostained with Vimentin-Booster (in green), cell nuclei were counterstained with DAPI (in blue).

Application

IF, ICC

Conjugate

ATTO 488

Specificity

Vimentin

Sizes
-_- / -_-

Cat No. vba488
×

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MDCK cells were immunostained with Vimentin-Booster (in green), cell nuclei were counterstained with DAPI (in blue).
EMT Essentials Antibody Kit

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PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Host

Rabbit/Mouse

Reactivity

Target Dependent

Applications

Target Dependent

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. PK30014
×

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PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Western blot analysis of MMP2 in various cell lines using Proteintech antibody 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP Result of anti-SNAI1 (IP:13099-1-AP, 4ug; Detection:13099-1-AP 1:600) with MCF-7 cells lysate 1040ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13099-1-AP (SNAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
EMT Expanded Antibody Kit

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PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Host

Rabbit/Mouse

Reactivity

Target Dependent

Applications

Target Dependent

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. PK30015
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Western blot analysis of MMP2 in various cell lines using Proteintech antibody 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66452-1-Ig (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IP Result of anti-SNAI1 (IP:13099-1-AP, 4ug; Detection:13099-1-AP 1:600) with MCF-7 cells lysate 1040ug.

IP Result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug.

IP Result of anti-TWIST1-specific (IP:25465-1-AP, 4ug; Detection:25465-1-AP 1:500) with MDA-MB-453s cells lysate 920ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13099-1-AP (SNAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP9 (N-terminal) antibody (10375-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (13050-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
VIM E.Coli expressed Tagged-proteins (2 total products)

2 Publications

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag28446, Ag0489
Vimentin VHHs (3 total products)

Applications

IF/ICC
-_- / -_-

Cat No. vt, vfms, vfrb
Vimentin-Chromobody® plasmid (TagGFP)

2 Publications

View More Images (2)

Vector Type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Selection

Kan/Neo

Sizes
-_- / -_-

Cat No. vcg
×

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More validation images available on the product page

Visualize vimentin cytoskeleton with the Vimentin-Chromobody in real time in your cell line of interest

Vimentin-Chromobody visualizes Vimentin filaments in live cells