Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, yellow), rabbit IgG anti-GM130 antibody (11308-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta) and rabbit IgG anti-Tubulin antibody (80713-1-RR) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, grey). Cell nuclei were stained with DAPI (blue).
Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, yellow), rabbit IgG anti-GM130 antibody (11308-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta) and rabbit IgG anti-Tubulin antibody (80713-1-RR) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, grey). Cell nuclei were stained with DAPI (blue).
Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-TDP43 antibody directly conjugated with CoraLite® Plus 488 (CL488-10782, green), rabbit IgG anti-GM130 antibody directly conjugated with CoraLite® Plus 555 (CL555-11308, red), rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta) and rabbit IgG anti-Tubulin antibody (80713-1-RR) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, cyan).
Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-TDP43 antibody directly conjugated with CoraLite® Plus 488 (CL488-10782, green), rabbit IgG anti-GM130 antibody directly conjugated with CoraLite® Plus 555 (CL555-11308, red), rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta) and rabbit IgG anti-Tubulin antibody (80713-1-RR) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, cyan).
Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-TDP43 antibody (10782-2-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), rabbit IgG anti-CoxIV antibody (11242-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, yellow), rabbit IgG anti-GM130 antibody (11308-1-AP) labeled with Multi-rAb CoraLite® Plus 647 Goat anti-rabbit Secondary (RGAR005, magenta) and rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, grey).
Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-TDP43 antibody (10782-2-AP ) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), rabbit IgG anti-CoxIV antibody (11242-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, yellow), rabbit IgG anti-GM130 antibody (11308-1-AP) labeled with Multi-rAb CoraLite® Plus 647 Goat anti-rabbit Secondary (RGAR005, magenta) and rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, grey).
Immunofluorescence of Hela cells: PFA-fixed cells were stained with rabbit IgG anti-Lamin antibody (12987-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504, grey). Cell nuclei were stained with DAPI (blue).
Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
1X10^6 HeLa cells were intracellularly stained with anti-Mitofilin rabbit polyclonal antibody (10179-1-AP, red) or with isotype control antibody (30000-0-AP, blue) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504). Cells were fixed and permeabilized with Foxp3 /Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HeLa cells were intracellularly stained with anti-TOM20 rabbit polyclonal antibody (11802-1-AP, orange) or with isotype control antibody (30000-0-AP, blue) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA504). Cells were fixed and permeabilized with Foxp3 /Transcription Factor Staining Buffer Kit (PF00011).