HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.
IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:2000) with the vector plasmid pEGFP-N1 Transfected HEK-293 cells lysate 300ug.
Immunofluorescent analysis of Transfected HEK-293 cells using 66002-1-Ig (GFP tag antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).
