Results for "hedgehog" | Proteintech Group

17 product results for "hedgehog"

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HumanKine® recombinant human Sonic Hedgehog (SHH) protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

2 Publications

Reviews (3)

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Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

Typically ≤350 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>90%

Sizes
-_- / -_-

Cat No. HZ-1306
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Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL. 
Shh Recombinant antibodies (240057E7) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse, rat

Applications

ELISA, IF/ICC, WB

Conjugation

CoraLite® Plus 750, Unconjugated

CloneNo.

240057E7
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Cat No. CL750-83280, 83280-1-RR, 83280-1-PBS
SHH Recombinant antibodies (240057G4) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

240057G4
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Cat No. 83280-2-PBS, 83280-2-RR
SHH Polyclonal antibody

KO/KD Validated

74 Publications

Reviews (1)

View More Images (10)

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Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, mink

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Sizes
-_- / -_-

Cat No. 20697-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.

IP result of anti-SHH (IP:20697-1-AP, 4ug; Detection:20697-1-AP 1:500) with mouse liver tissue lysate 6400ug.

Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HHIP Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50174-1	68807-1-PBS	68807-2-PBS	HHIP	Human	Cytometric bead array	CBA: 1.563-100 ng/mL

IHH Polyclonal antibody

9 Publications

Reviews (1)

View More Images (5)

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human kidney tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 13388-1-AP
×

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human kidney tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13388-1-AP (IHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
DHH Polyclonal antibody

3 Publications

Reviews (1)

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mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 13889-1-AP
×

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mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HHIP Polyclonal antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 29466-1-AP
×

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More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HHIP antibody (29466-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHCeasy IHH Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0135 (IHH IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
-_- / -_-

Cat No. KHC0135
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0135 (IHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0135 (IHH IHC Kit).
IHCeasy SHH Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0134 (SHH IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human, mouse

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
-_- / -_-

Cat No. KHC0134
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0134 (SHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC0134 (SHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC0134 (SHH IHC Kit).
SHH Polyclonal antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Sizes
-_- / -_-

Cat No. 29752-1-AP
×

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More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHH (29752-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Shh Polyclonal antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 30951-1-AP (SHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 30951-1-AP
×

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Various lysates were subjected to SDS PAGE followed by western blot with 30951-1-AP (SHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
DHH Fusion Protein

Enlarge Image

Species

human

Source

E. coli-derived, PET28a

Tag

6*His

Format

Powder

Sizes
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Cat No. Ag5212
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DHH fusion protein Ag5212 SDS-PAGE
SHH Fusion Protein

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Species

human

Source

E. coli-derived, PET28a

Tag

6*His

Format

Powder

Sizes
-_- / -_-

Cat No. Ag29133
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SHH fusion protein Ag29133 SDS-PAGE