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HumanKine® recombinant human IL-5 protein

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Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.2-0.9 ng/mL

Expression

HEK293

Reactivity

human

Purity

>95%

Sizes
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Cat No. HZ-1324
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Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IL-5 (HZ-1324) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-5 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-0.9 ng/mL.
IL-5 Polyclonal antibody

3 Publications

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, IHC, ELISA

Sizes
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Cat No. 26677-1-AP
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Recombinant protein were subjected to SDS PAGE followed by western blot with 26677-1-AP (IL5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL5 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00921-1	83963-1-PBS	83963-2-PBS	IL-5	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP50091-1	68737-1-PBS	68738-1-PBS	IL5	human	Sandwich ELISA	sELISA: 0.977-250 ng/mL

Mouse IL-5 ELISA Kit

9 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Sizes
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Cat No. KE10018
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Human IL-5 ELISA Kit

3 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell Culture supernatants, Urine

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE00217
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Human IL-5 ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant, Urine

Sensitivity

0.42 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE00304
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Standard curve of Human IL-5 ELISA Kit KE00304.
IL-5 Polyclonal antibody

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Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 30198-1-AP (IL-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 30198-1-AP
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Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 30198-1-AP (IL-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IL-5 Fusion Protein

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Species

human

Source

E. coli-derived, PET30a

Tag

6*His

Format

Powder

Sizes
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Cat No. Ag24664
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IL-5 fusion protein Ag24664 SDS-PAGE
IL-5RA Polyclonal antibody

2 Publications

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human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 12655-1-AP
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human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human IL-5 protein

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IL-5 Polyclonal antibody

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IL5 Matched Antibody Pairs^New

Mouse IL-5 ELISA Kit

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Human IL-5 ELISA Kit

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Human IL-5 ELISA Kit

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IL-5 Polyclonal antibody

-_- / -_-

IL-5 Fusion Protein

-_- / -_-

IL-5RA Polyclonal antibody

-_- / -_-

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human IL-5 protein

-_- / -_-

IL-5 Polyclonal antibody

-_- / -_-

IL5 Matched Antibody PairsNew

Mouse IL-5 ELISA Kit

-_- / -_-

Human IL-5 ELISA Kit

-_- / -_-

Human IL-5 ELISA Kit

-_- / -_-

IL-5 Polyclonal antibody

-_- / -_-

IL-5 Fusion Protein

-_- / -_-

IL-5RA Polyclonal antibody

-_- / -_-

Products derived from

IL5 Matched Antibody Pairs^New