Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Recombinant protein were subjected to SDS PAGE followed by western blot with 26677-1-AP (IL5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human IL-5 (HZ-1324) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-5 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-0.9 ng/mL.
