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77 product results for:

"il-6"

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IL-6 Polyclonal antibody

KO/KD Validated

575 Publications

Reviews (2)

View More Images (2)

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Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

bovine, canine, goat, human and More (3)

Applications

WB, FC, IHC, ELISA, Neutralization, Cell treatment

Sizes
-_- / -_-

Cat No. 21865-1-AP
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Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 HUVEC cells untreated (dashed lines), LPS and Brefeldin A treated (red) were intracellularly stained with 0.5 ug Anti-Human IL-6 (21865-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.
IL-6 Monoclonal antibody

KO/KD Validated

176 Publications

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Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG2b

Reactivity

human, pig, human

Applications

WB, IF, IHC, ELISA

CloneNo.

1A3B4

Sizes
-_- / -_-

Cat No. 66146-1-Ig
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Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Non-treated, LPS and protein transport inhibitor treated HUVEC cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IL-6 Recombinant antibody

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HUVEC cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

Human

Applications

WB, ELISA

CloneNo.

2E9

Sizes
-_- / -_-

Cat No. 81077-1-RR
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HUVEC cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HumanKine® recombinant human IL-6 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

41 Publications

Reviews (1)

View More Images (3)

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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.03-0.24 ng/mL in 3G12B10 cells.

Expression

HEK293

Reactivity

human, mouse, monkey, rabbit and More (1)

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1019
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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL. 

Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL.
IL-6 Polyclonal antibody

13 Publications

Enlarge Image

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untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 26404-1-AP
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untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
NeutraControl IL-6 Monoclonal antibody

1 Publications

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Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis).

Host/IsoType

Mouse/IgG1

Reactivity

human, Human

Applications

Non-Neutralization, ELISA

CloneNo.

1E9B8

Sizes
-_- / -_-

Cat No. 69501-1-Ig
×

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Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human IL-6 (Cat.NO. HZ-1019) at 70 ng/well followed by blocking and adding serial diluted IL-6 antibody 69001-1-Ig and 69501-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
IL-6 Hybrid Matched Antibody Pair, PBS Only

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Applications

Cytometric bead array, Sandwich ELISA

SandwichRange

1.563-100 ng/mL

Sizes
-_- / -_-

Cat No. MP80002-1
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Standard curve of MP80002-1

Standard curve of MP80002-1
IL-6 Monoclonal antibody, PBS Only

KO/KD Validated

View More Images (2)

×
Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

Host/IsoType

Mouse/IgG2b

Reactivity

human

Applications

WB, ELISA

CloneNo.

1A3B4

Sizes
-_- / -_-

Cat No. 66146-1-PBS
×

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Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

Non-treated, LPS and protein transport inhibitor treated HUVEC cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.
IL6 Monoclonal Matched Antibody Pair, PBS Only

Enlarge Image

Applications

Sandwich ELISA

SandwichRange

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. MP50032-1
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Standard curve of MP50032-1

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

77 product results for:

"il-6"

IL-6 Polyclonal antibody

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IL-6 Monoclonal antibody

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IL-6 Recombinant antibody

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HumanKine® recombinant human IL-6 protein

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IL-6 Polyclonal antibody

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NeutraControl IL-6 Monoclonal antibody

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IL-6 Hybrid Matched Antibody Pair, PBS Only

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IL-6 Monoclonal antibody, PBS Only

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IL6 Monoclonal Matched Antibody Pair, PBS Only

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