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100 product results for "il-6"

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HumanKine® recombinant human IL-6 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

47 Publications

Reviews (1)

View More Images (3)

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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.03-0.24 ng/mL in 3G12B10 cells.

Expression

HEK293

Reactivity

human, mouse, rat, monkey, rabbit

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1019
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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL. 

Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL.
HumanKine® recombinant human IL-6 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.03-0.24 ng/mL

Expression

HEK293

Reactivity

human, monkey

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1019-GMP
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Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-6 (HZ-1019-GMP) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of GMP recombinant IL-6 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.03-0.24 ng/mL.
IL-6 Recombinant antibodies (240670F10) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

IF/ICC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

240670F10
-_- / -_-

Cat No. 83747-5-RR, 83747-5-PBS, CL488-83747-5
IL-6 Polyclonal antibody

KO/KD Validated

632 Publications

Reviews (2)

View More Images (3)

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Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, canine, goat, pig, rabbit

Applications

WB, IHC, IP, Neutralization, ELISA

Sizes
-_- / -_-

Cat No. 21865-1-AP
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Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-IL-6 (IP:21865-1-AP, 4ug; Detection:21865-1-AP 1:1000) with LPS and Protein transport inhibitor treated HUVEC cells lysate 800 ug.

Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (21865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IL-6 Monoclonal antibodies (1A3B4) (2 total products)

KO/KD Validated

206 Publications

Host/IsoType

Mouse/IgG2b

Reactivity

human, pig, sheep

Applications

IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

1A3B4
-_- / -_-

Cat No. 66146-1-PBS, 66146-1-Ig

IL-6 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50032-1	68659-1-PBS	68659-2-PBS	IL-6	human	Cytometric bead array, Sandwich ELISA	sELISA: 7.8-500 pg/mL, CBA: 0.098-12.5 ng/mL
MP00264-1	83179-1-PBS	83179-3-PBS	IL-6	Rat	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00264-2	83179-2-PBS	83179-3-PBS	IL-6	Rat	Cytometric bead array, Sandwich ELISA	sELISA: 62.5-4000 pg/mL, CBA: 0.625-20 ng/mL
MP00264-3	83179-2-PBS	83179-1-PBS	IL-6	Rat	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00687-1	83747-2-PBS	83747-4-PBS	IL-6	Human	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00687-2	83747-2-PBS	83747-1-PBS	IL-6	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00687-3	83747-2-PBS	83747-3-PBS	IL-6	Human	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00687-4	83747-2-PBS	83747-6-PBS	IL-6	Human	Sandwich ELISA	sELISA: 7.8-500 pg/mL
MP00756-1	83795-1-PBS	83795-3-PBS	IL-6	Mouse	Cytometric bead array	CBA: 1.25-40 ng/mL
MP00756-2	83795-4-PBS	83795-2-PBS	IL-6	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00756-3	83795-5-PBS	83795-2-PBS	IL-6	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00756-4	83795-6-PBS	83795-1-PBS	IL-6	Mouse	Sandwich ELISA	sELISA: 15.6-1000 pg/mL
MP50032-2	68659-3-PBS	66146-2-PBS	IL-6	Human	Cytometric bead array	CBA: 1.563-100 ng/mL
MP50032-3	68659-13-PBS	68659-10-PBS	IL-6	Human	Cytometric bead array	CBA: 3.125-50 ng/mL
MP80002-1	81077-1-PBS	68659-10-PBS	IL-6	Human	Cytometric bead array	CBA: 1.563-100 ng/mL

Mouse IL-6 ELISA Kit

312 Publications

Reviews (4)

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

3.8 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE10007
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IL-6 Recombinant antibodies (2E9) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, ELISA, WB

Conjugation

Unconjugated

CloneNo.

2E9
-_- / -_-

Cat No. 81077-1-RR, 81077-1-PBS
AuthentiKine™ Human IL-6 ELISA Kit

79 Publications

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1.2 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE00139
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IL-6 Polyclonal antibody

24 Publications

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untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse, rat, bovine, chicken

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 26404-1-AP
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untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Mouse IL-6 ELISA Kit

10 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

4.0 pg/mL

Range

12.5-800 pg/mL

Sizes
-_- / -_-

Cat No. KE10091
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NeutraKine® IL-6 Monoclonal antibodies (4G3E6) (2 total products)

10 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, rat

Applications

ELISA, IF/ICC, IHC, Neutralization & Neutro-controls

Conjugation

Unconjugated

CloneNo.

4G3E6
-_- / -_-

Cat No. 69001-1-Ig, 69001-1-PBS
Rat IL-6 ELISA Kit

2 Publications

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

29.8 pg/mL

Range

62.5-4000 pg/mL

Sizes
-_- / -_-

Cat No. KE20024
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Standard Curve of KE20024 Rat IL-6 ELISA Kit

Standard Curve of KE20024 Rat IL-6 ELISA Kit
NeutraControl IL-6 Monoclonal antibody

1 Publications

View More Images (2)

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Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Non-Neutralization

CloneNo.

1E9B8

Sizes
-_- / -_-

Cat No. 69501-1-Ig
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Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human IL-6 (Cat.NO. HZ-1019) at 70 ng/well followed by blocking and adding serial diluted IL-6 antibody 69001-1-Ig and 69501-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
anti-IL6 recombinant VHH, biotinylated

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BLI analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

Application

BLI, SPR, ELISA

Conjugate

Biotin

Reactivity

human

Sizes
-_- / -_-

Cat No. IL6-b
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BLI analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

ELISA analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Detection via rabbit polyclonal PTG 21865-1-AP.
anti-IL6 recombinant VHH, for 1x Cys conjugation

Enlarge Image

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BLI analysis of the interaction between varying concentrations of anti-IL-6 VHH and immobilised, biotinylated HumanKine IL-6 (HZ-1019). Fit indicates fitting of data to a 1:1 binding model.

Application

Conjugation

Conjugate

Unconjugated

Reactivity

human

Sizes
-_- / -_-

Cat No. IL6Cys1
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BLI analysis of the interaction between varying concentrations of anti-IL-6 VHH and immobilised, biotinylated HumanKine IL-6 (HZ-1019). Fit indicates fitting of data to a 1:1 binding model.
Human IL-6 ELISA Kit

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell Culture Supernatant

Sensitivity

0.2 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE00385
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Standard Curve of KE00385 Human IL-6 ELISA Kit

Standard Curve of KE00385 Human IL-6 ELISA Kit
IL-6 Polyclonal antibody

Enlarge Image

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LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 29444-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 29444-1-AP
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LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 29444-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IL-6 Polyclonal antibody

Enlarge Image

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LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 30953-1-AP (IL-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

rat

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 30953-1-AP
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LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 30953-1-AP (IL-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IL-6 Recombinant antibodies (240670D12) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

240670D12
-_- / -_-

Cat No. 98087-1-RR, 98087-1-PBS
IL-6 Recombinant antibodies (240701E8) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

240701E8
-_- / -_-

Cat No. 98089-1-PBS, 98089-1-RR

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human IL-6 protein

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HumanKine® recombinant human IL-6 protein- GMP grade

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IL-6 Recombinant antibodies (240670F10) (3 total products)

-_- / -_-

IL-6 Polyclonal antibody

-_- / -_-

IL-6 Monoclonal antibodies (1A3B4) (2 total products)

-_- / -_-

IL-6 Matched Antibody PairsNew

Mouse IL-6 ELISA Kit

-_- / -_-

IL-6 Recombinant antibodies (2E9) (2 total products)

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AuthentiKine™ Human IL-6 ELISA Kit

-_- / -_-

IL-6 Polyclonal antibody

-_- / -_-

Mouse IL-6 ELISA Kit

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NeutraKine® IL-6 Monoclonal antibodies (4G3E6) (2 total products)

-_- / -_-

Rat IL-6 ELISA Kit

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NeutraControl IL-6 Monoclonal antibody

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anti-IL6 recombinant VHH, biotinylated

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anti-IL6 recombinant VHH, for 1x Cys conjugation

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Human IL-6 ELISA Kit

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IL-6 Polyclonal antibody

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IL-6 Polyclonal antibody

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IL-6 Recombinant antibodies (240670D12) (2 total products)

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IL-6 Recombinant antibodies (240701E8) (2 total products)

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Products derived from

IL-6 Matched Antibody Pairs^New