Results

49 product results for "mTOR"

Filters

mTOR Monoclonal antibodies (1G11A3) (3 total products)

KO/KD Validated

373 Publications

Reviews (3)

Host/IsoType

Mouse/IgG2a

Reactivity

human, mouse, rat, chicken, goat, hamster, pig, zebra finches

Applications

ELISA, IF/ICC, IHC, IP, WB

Conjugation

CoraLite®594, Unconjugated

CloneNo.

1G11A3
-_- / -_-

Cat No. CL594-66888, 66888-1-Ig, 66888-1-PBS
mTOR Recombinant antibodies (6H23) (2 total products)

KO/KD Validated

1 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

Unconjugated

CloneNo.

6H23
-_- / -_-

Cat No. 81670-1-RR, 81670-1-PBS
mTOR Polyclonal antibodies (2 total products)

KO/KD Validated

118 Publications

Reviews (1)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, chicken, monkey, rabbit

Applications

FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 28273-1-AP, CL488-28273
Phospho-mTOR (Ser2448) Monoclonal antibodies (2A12G3) (5 total products)

284 Publications

Reviews (1)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, bovine, chicken, pig

Applications

FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®594, Unconjugated

CloneNo.

2A12G3
-_- / -_-

Cat No. 67778-1-PBS, 67778-1-Ig, CL488-67778, CL647-67778, CL594-67778
Phospho-mTOR (Ser2448) Recombinant antibodies (3L18) (6 total products)

22 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine

Applications

ELISA, FC (Intra), IF/ICC, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®555, CoraLite®594, Unconjugated

CloneNo.

3L18
-_- / -_-

Cat No. CL488-80596, CL647-80596, CL555-80596, 80596-1-PBS, CL594-80596, 80596-1-RR

mTOR Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50209-1	66888-2-PBS	66888-3-PBS	mTOR	Human	Sandwich ELISA	sELISA: 0.049-50 ng/mL
MP50209-2	66888-2-PBS	66888-4-PBS	mTOR	Human	Sandwich ELISA	sELISA: 0.049-50 ng/mL

Phospho-mTOR (Ser2481) Polyclonal antibody

9 Publications

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Nontreated and insulin-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28879-1-AP (Phospho-mTOR (Ser2481) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 28879-1-AP
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Nontreated and insulin-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28879-1-AP (Phospho-mTOR (Ser2481) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Phospho-mTOR (Ser2448) Polyclonal antibody

2 Publications

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Non-treated and Rapamycin treated lysates were subjected to SDS PAGE followed by western blot with 28881-1-AP (Phospho-mTOR (Ser2448) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (28273-1-AP) and Vinculin (66305-1-Ig) subsequently.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 28881-1-AP
×

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Non-treated and Rapamycin treated lysates were subjected to SDS PAGE followed by western blot with 28881-1-AP (Phospho-mTOR (Ser2448) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (28273-1-AP) and Vinculin (66305-1-Ig) subsequently.
IHCeasy MTOR Ready-To-Use IHC Kit

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Primary Ab Type

Mouse Monoclonal

Reactivity

human, mouse, rat

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC2260
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Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using KHC2260 (MTOR IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using KHC2260 (MTOR IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC2260 (MTOR IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using KHC2260 (MTOR IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using KHC2260 (MTOR IHC Kit).
AKT Substrate Essentials Antibody Kit

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Non-treated and Calyculin A treated cell lysates were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Host

Rabbit/Mouse

Reactivity

Target Dependent

Applications

Target Dependent

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. PK30021
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Non-treated and Calyculin A treated cell lysates were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80698-1-RR (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control.

Non-treated MCF-7 cells and Cobalt Chloride treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 28757-1-AP (Phospho-FOXO1 (Ser319) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67558-1-Ig (GSK3B-phospho-S9 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using Phospho-GSK3B (Ser9) antibody (67558-1-Ig, Clone: 1C9E2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed PMA treated HEK-293 cells using Phospho-mTOR (Ser2448) antibody (80596-1-RR, Clone: 3L18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
mTOR Substrates Antibody Kit

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Various lysates were subjected to SDS PAGE followed by western blot with 81670-1-RR (mTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host

Rabbit

Reactivity

Target Dependent

Applications

Target Dependent

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. PK30025
×

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Various lysates were subjected to SDS PAGE followed by western blot with 81670-1-RR (mTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB result of mTOR antibody (81670-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently.

Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82373-1-RR (Phospho-p70(S6K) (Thr389) antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control.

Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Non-treated and PDGF treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (RPS6-phospho-S235/236 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

IP result of anti-mTOR (IP:81670-1-RR, 4ug; Detection:81670-1-RR 1:1000) with HeLa cells lysate 1760 ug.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81670-1-RR (mTOR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using mTOR antibody (81670-1-RR, Clone: 6H23 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed PMA treated HEK-293 cells using Phospho-mTOR (Ser2448) antibody (80596-1-RR, Clone: 3L18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human mTOR (81670-1-RR, Clone:6H23) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Recombinant human MTOR protein

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Species

human

Source

E. coli-derived, PET28a

Tag

6*His

Format

Powder

Sizes
-_- / -_-

Cat No. Ag28395
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MTOR fusion protein Ag28395 SDS-PAGE
Raptor Polyclonal antibody

KO/KD Validated

45 Publications

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Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, IP, ELISA, PLA

Sizes
-_- / -_-

Cat No. 20984-1-AP
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WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug.

Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
RICTOR Polyclonal antibody

KO/KD Validated

24 Publications

Reviews (1)

View More Images (7)

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Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Sizes
-_- / -_-

Cat No. 27248-1-AP
×

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Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (27248-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RICTOR antibody (27248-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
HBXIP Polyclonal antibody

KO/KD Validated

11 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 14492-1-AP (HBXIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Sizes
-_- / -_-

Cat No. 14492-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 14492-1-AP (HBXIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14492-1-AP (HBXIP Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14492-1-AP (HBXIP Antibody) at dilution of 1:200 (under 40x lens).

1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug HBXIP Polyclonal antibody (14492-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IP result of anti-HBXIP (IP:14492-1-AP, 4ug; Detection:14492-1-AP 1:300) with K-562 cells lysate 1600 ug.

RICTOR Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50234-1	66867-2-PBS	66867-3-PBS	RICTOR	Human	Cytometric bead array	CBA: 0.781-100 ng/mL

RICTOR Monoclonal antibodies (1D1C4) (2 total products)

4 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat

Applications

Cytometric bead array, ELISA, IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

1D1C4
-_- / -_-

Cat No. 66867-2-PBS, 66867-2-Ig
MAPKSP1 Polyclonal antibody

1 Publications

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human liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 11937-1-AP
×

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human liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).
Raptor Monoclonal antibodies (1B8A12) (2 total products)

1 Publications

Host/IsoType

Mouse/IgG2b

Reactivity

human

Applications

ELISA, IP, WB

Conjugation

Unconjugated

CloneNo.

1B8A12
-_- / -_-

Cat No. 68286-1-PBS, 68286-1-Ig
LAMTOR1 Polyclonal antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 29611-1-AP (C11orf59,LAMTOR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 29611-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 29611-1-AP (C11orf59,LAMTOR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using LAMTOR1 antibody (29611-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
Phospho-RICTOR (Thr1135) Polyclonal antibody

Enlarge Image

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Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29117-1-AP (Phospho-RICTOR (Thr1135) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 29117-1-AP
×

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More validation images available on the product page

Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29117-1-AP (Phospho-RICTOR (Thr1135) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

mTOR Monoclonal antibodies (1G11A3) (3 total products)

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mTOR Recombinant antibodies (6H23) (2 total products)

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mTOR Polyclonal antibodies (2 total products)

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Phospho-mTOR (Ser2448) Monoclonal antibodies (2A12G3) (5 total products)

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Phospho-mTOR (Ser2448) Recombinant antibodies (3L18) (6 total products)

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mTOR Matched Antibody PairsNew

Phospho-mTOR (Ser2481) Polyclonal antibody

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Phospho-mTOR (Ser2448) Polyclonal antibody

-_- / -_-

IHCeasy MTOR Ready-To-Use IHC Kit

-_- / -_-

AKT Substrate Essentials Antibody Kit

-_- / -_-

mTOR Substrates Antibody Kit

-_- / -_-

Recombinant human MTOR protein

-_- / -_-

Raptor Polyclonal antibody

-_- / -_-

RICTOR Polyclonal antibody

-_- / -_-

HBXIP Polyclonal antibody

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RICTOR Matched Antibody PairsNew

RICTOR Monoclonal antibodies (1D1C4) (2 total products)

-_- / -_-

MAPKSP1 Polyclonal antibody

-_- / -_-

Raptor Monoclonal antibodies (1B8A12) (2 total products)

-_- / -_-

LAMTOR1 Polyclonal antibody

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Phospho-RICTOR (Thr1135) Polyclonal antibody

-_- / -_-

Products derived from

mTOR Matched Antibody Pairs^New

RICTOR Matched Antibody Pairs^New