Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB result of Cytochrome c antibody (10993-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.
WB result of Cytochrome c antibody (10993-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cytochrome c (10993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (11242-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB result of COXIV antibody (11242-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells.
IP result of anti-COXIV (IP:11242-1-AP, 3ug; Detection:11242-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3500ug.
Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/2 Polyclonal antibody (10866-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/2 antibody (10866-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of VDAC1 antibody (55259-1-AP, 1:2000) with si-Control and si-VDAC1 transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human VDAC1/Porin (55259-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP result of anti-HSP60 (IP:15282-1-AP, 4ug; Detection:15282-1-AP 1:10000) with HEK-293 cells lysate 1800 ug.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (15282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP60 (15282-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various cells were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (66264-1-Ig, Clone: 2D8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).
WB result of Cytochrome c antibody (66264-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Cytochrome c (66264-1-Ig, Clone:2D8D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.
HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB result of LONP1 antibody (15440-1-AP, 1:6000) with si-Control and si-LONP1 transfected Jurkat cells.
Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP result of anti-LONP1 (IP:15440-1-AP, 3ug; Detection:15440-1-AP 1:1000) with mouse heart tissue lysate 9500ug.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Various lysates were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IP result of anti-Mitofilin (IP:10179-1-AP, 3ug; Detection:10179-1-AP 1:1000) with Raji cells lysate 3800ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10179-1-AP (Mitofilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mitofilin antibody (10179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Mitofilin (10179-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells.
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Biolayer interferometry (BLl) kinetic assays of 82916-1-RR
against Human COXIV were performed. The affinity constant is 9.41 nM.
WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 82916-1-RR
against Human COXIV were performed. The affinity constant is 9.41 nM.
Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB result of Cytochrome c antibody (83276-1-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (83276-1-RR, Clone: 230503E10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Biolayer interferometry (BLl) kinetic assays of 83276-1-RR against Human Cytochrome c were performed. The affinity constant is below 1 pM.
Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.
WB result of Cytochrome c antibody (83276-1-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (83276-1-RR, Clone: 230503E10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 83276-1-RR against Human Cytochrome c were performed. The affinity constant is below 1 pM.
Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of anti-IDH2(IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.
IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 81538-1-RR (VDAC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VDAC1/Porin Recombinant antibody (81538-1-RR, Clone:2F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VDAC1/Porin antibody (81538-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 81538-1-RR (VDAC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VDAC1/Porin Recombinant antibody (81538-1-RR, Clone:2F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VDAC1/Porin antibody (81538-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81538-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11081-1-AP (UCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 83552-2-RR (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
1x10^6 U-251 cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-2-RR, Clone:240460G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Biolayer interferometry (BLl) kinetic assays of 83552-2-RR against Human LONP1 were performed. The affinity constant is 1.76 nM.
Various lysates were subjected to SDS PAGE followed by western blot with 83552-2-RR (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.
1x10^6 U-251 cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-2-RR, Clone:240460G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.
