Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.
Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.
Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.
ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.
