Results

375 product results for "necroptosis"

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HumanKine® recombinant human TNF alpha protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

52 Publications

Reviews (2)

View More Images (3)

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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.002-0.026 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1014
×

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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL. 

Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.
HumanKine® recombinant human TNF alpha protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

View More Images (2)

×
Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.002-0.026 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1014-GMP
×

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More validation images available on the product page

Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human TNF alpha (HZ-1014-GMP) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.002-0.026 ng/mL.
Caspase 9/p35/p10 Polyclonal antibodies (4 total products)

KO/KD Validated

770 Publications

Reviews (2)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, duck, goat, hamster, pig, rabbit, sheep, zebrafish

Applications

FC (Intra), IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®555, Unconjugated
-_- / -_-

Cat No. CL488-10380, CL555-10380, 10380-1-AP, CL647-10380
TNF-alpha Polyclonal antibody

669 Publications

Reviews (2)

Enlarge Image

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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, fish, rabbit, sheep

Applications

WB, ELISA, Cell treatment, Transwell Assay

Sizes
-_- / -_-

Cat No. 17590-1-AP
×

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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
c-MYC Polyclonal antibodies (4 total products)

KO/KD Validated

657 Publications

Reviews (4)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, p. lilacinum, pig, sheep

Applications

ChIP, CoIP, FC (Intra), IF/ICC, IP, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®555, Unconjugated
-_- / -_-

Cat No. CL647-10828, CL488-10828, 10828-1-AP, CL555-10828
TLR4 Polyclonal antibody

KO/KD Validated

516 Publications

Reviews (1)

View More Images (3)

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mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, canine, chicken, hamster, pig, rabbit

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA, Microtiter plate binding assay

Sizes
-_- / -_-

Cat No. 19811-1-AP
×

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mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).
TNF-alpha Monoclonal antibodies (7B8A11) (4 total products)

KO/KD Validated

469 Publications

Reviews (2)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, bovine, chicken, duck, monkey, pig, rabbit

Applications

ELISA, IF-P, IHC, RIP, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

7B8A11
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Cat No. CL488-60291, 60291-1-PBS, CL594-60291, 60291-1-Ig
GSK3B Polyclonal antibodies (2 total products)

KO/KD Validated

352 Publications

Reviews (1)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, chicken, hamster, pig, zebrafish

Applications

CoIP, IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 22104-1-AP, CL488-22104
Caspase 8/p43/p18 Polyclonal antibodies (2 total products)

KO/KD Validated

331 Publications

Reviews (2)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, hamster, pig, rabbit

Applications

CoIP, FC (Intra), IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 13423-1-AP, CL488-13423
Phospho-JNK (Tyr185) Recombinant antibodies (2G8) (2 total products)

202 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, pig, sheep

Applications

ELISA, IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

2G8
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Cat No. 80024-1-RR, 80024-1-PBS
MYD88 Polyclonal antibody

KO/KD Validated

195 Publications

Reviews (2)

View More Images (5)

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Various lysates were subjected to SDS PAGE followed by western blot with 23230-1-AP (MYD88 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, canine, chicken, monkey, pig, sheep

Applications

WB, IHC, IF, IP, CoIP, ELISA

Sizes
-_- / -_-

Cat No. 23230-1-AP
×

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More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with 23230-1-AP (MYD88 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

WB result of MYD88 antibody (23230-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYD88 transfected HepG2 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 23230-1-AP (MYD88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IP result of anti-MYD88 (IP:23230-1-AP, 4ug; Detection:23230-1-AP 1:10000) with K-562 cells lysate 1360 ug.

IP result of anti-MYD88 (IP:23230-1-AP, 4ug; Detection:23230-1-AP 1:10000) with A549 cells lysate 640 ug.
JNK Polyclonal antibodies (2 total products)

158 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, pig

Applications

CoIP, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 24164-1-AP, CL488-24164
TLR4 Monoclonal antibodies (3G9A4) (2 total products)

KO/KD Validated

153 Publications

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, pig, rabbit, sheep, zebrafish

Applications

CoIP, IF/ICC, IHC, IP, WB

Conjugation

Unconjugated

CloneNo.

3G9A4
-_- / -_-

Cat No. 66350-1-PBS, 66350-1-Ig
JNK Monoclonal antibodies (1A12E1) (4 total products)

KO/KD Validated

147 Publications

Reviews (1)

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat, pig, rabbit, sheep

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

1A12E1
-_- / -_-

Cat No. 66210-1-Ig, CL488-66210, CL594-66210, 66210-1-PBS
c-MYC Recombinant antibodies (2B17) (3 total products)

KO/KD Validated

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

2B17
-_- / -_-

Cat No. 80845-1-RR, 80845-1-PBS, CL488-80845
GSK3B Recombinant antibodies (4N21) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, IHC, IP, WB

Conjugation

Unconjugated

CloneNo.

4N21
-_- / -_-

Cat No. 82061-1-RR, 82061-1-PBS
JNK Recombinant antibodies (6J16) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

6J16
-_- / -_-

Cat No. 81629-1-PBS, CL488-81629, 81629-1-RR
RIP3 Polyclonal antibodies (2 total products)

130 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, pig, yeast

Applications

CoIP, IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 17563-1-AP, CL488-17563
JNK Polyclonal antibody

123 Publications

View More Images (3)

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4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, pig

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Sizes
-_- / -_-

Cat No. 51151-1-AP
×

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More validation images available on the product page

4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Fas/CD95 Polyclonal antibody

KO/KD Validated

122 Publications

Reviews (2)

View More Images (3)

Host/IsoType

Rabbit/IgG

Reactivity

human, hamster, monkey, sheep

Applications

WB, IHC, IF, IP, CoIP, CHIP, ELISA

Sizes
-_- / -_-

Cat No. 13098-1-AP
×

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More validation images available on the product page

WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells.

Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug.
AURKA Recombinant antibodies (230087H3) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, IF/ICC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

230087H3
-_- / -_-

Cat No. CL488-82906, 82906-1-RR, 82906-1-PBS

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human TNF alpha protein

-_- / -_-

HumanKine® recombinant human TNF alpha protein- GMP grade

-_- / -_-

Caspase 9/p35/p10 Polyclonal antibodies (4 total products)

-_- / -_-

TNF-alpha Polyclonal antibody

-_- / -_-

c-MYC Polyclonal antibodies (4 total products)

-_- / -_-

TLR4 Polyclonal antibody

-_- / -_-

TNF-alpha Monoclonal antibodies (7B8A11) (4 total products)

-_- / -_-

GSK3B Polyclonal antibodies (2 total products)

-_- / -_-

Caspase 8/p43/p18 Polyclonal antibodies (2 total products)

-_- / -_-

Phospho-JNK (Tyr185) Recombinant antibodies (2G8) (2 total products)

-_- / -_-

MYD88 Polyclonal antibody

-_- / -_-

JNK Polyclonal antibodies (2 total products)

-_- / -_-

TLR4 Monoclonal antibodies (3G9A4) (2 total products)

-_- / -_-

JNK Monoclonal antibodies (1A12E1) (4 total products)

-_- / -_-

c-MYC Recombinant antibodies (2B17) (3 total products)

-_- / -_-

GSK3B Recombinant antibodies (4N21) (2 total products)

-_- / -_-

JNK Recombinant antibodies (6J16) (3 total products)

-_- / -_-

RIP3 Polyclonal antibodies (2 total products)

-_- / -_-

JNK Polyclonal antibody

-_- / -_-

Fas/CD95 Polyclonal antibody

-_- / -_-

AURKA Recombinant antibodies (230087H3) (3 total products)

-_- / -_-

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