A431 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
mouse colon tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).