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77 product results for "pdgf"

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HumanKine® recombinant human PDGFbb protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

11 Publications

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Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.3-3 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1308
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Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human PDGFbb (HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 48 hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.3-3 ng/mL.
HumanKine® recombinant human PDGFaa protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

6 Publications

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Purity of recombinant human PDGFaa was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

Typically ≤ 10 ng/mL in 3T3 cell, 150 - 900 ng/mL in NIH 3T3

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1215
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Purity of recombinant human PDGFaa was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human PDGFaa (HZ-1215) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFaa for 48hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values are less than 10 ng/mL EC50. 
HumanKine® recombinant human PDGFbb protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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×
Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.3-3 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1308-GMP
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Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human PDGFbb (HZ-1308-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.3-3 ng/mL.
PDGFB Recombinant antibodies (230486A7) (2 total products)

2 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, IHC, WB

Conjugation

Unconjugated

CloneNo.

230486A7
-_- / -_-

Cat No. 83214-5-RR, 83214-5-PBS
PDGF-D Polyclonal antibodies (2 total products)

KO/KD Validated

14 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, goat

Applications

IF/ICC, IF-P, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 14075-1-AP, CL488-14075

PDGFB Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00198-1	83214-2-PBS	83214-3-PBS	PDGFB	human	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00198-2	83214-4-PBS	83214-3-PBS	PDGFB	human	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00198-3	83214-1-PBS	83214-2-PBS	PDGFB	human	Cytometric bead array	CBA: 0.625-20 ng/mL

Human PDGF-BB ELISA Kit

8 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

0.5 pg/mL

Range

3.9-250 pg/mL

Sizes
-_- / -_-

Cat No. KE00161
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NeutraKine® PDGF-BB Monoclonal antibody

2 Publications

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Recombinant human PDGFbb (Cat.NO. HZ-1308) stimulates proliferation of BALB/3T3 (mouse fibroblast cell line) in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGFbb (20ng/mL HZ-1308) is neutralized by mouse anti-human PDGFbb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL.

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Neutralization, Cell treatment

CloneNo.

4G11C7

Sizes
-_- / -_-

Cat No. 69020-1-Ig
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Recombinant human PDGFbb (Cat.NO. HZ-1308) stimulates proliferation of BALB/3T3 (mouse fibroblast cell line) in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGFbb (20ng/mL HZ-1308) is neutralized by mouse anti-human PDGFbb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL.

Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
NeutraControl PDGF-BB Monoclonal antibody

1 Publications

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Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

Host/IsoType

Mouse/IgG2a

Reactivity

human

Applications

ELISA, Non-Neutralization

CloneNo.

4E2C7

Sizes
-_- / -_-

Cat No. 69520-1-Ig
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Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
Human PDGF-AB ELISA Kit

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

14.5 pg/mL

Range

31.25 - 1000 pg/mL

Sizes
-_- / -_-

Cat No. KE00222
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Standard curve of Human PDGF-AB ELISA Kit KE00222.
PDGFA Polyclonal antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 26940-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
PDGFC Polyclonal antibody

3 Publications

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MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 55076-1-AP
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MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
PDGFA E.Coli expressed Tagged-proteins (3 total products)

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag17993, Ag26228, Ag25643
PDGFB E.Coli expressed Tagged-proteins (2 total products)

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag14468, Ag24502
PDGFD E.Coli expressed Tagged-proteins (3 total products)

Species

human

Source

PET20b, PET28a

Tag

6*His
-_- / -_-

Cat No. Ag5154, Ag29199, Ag7430
PDGFR beta Recombinant antibodies (230214D11) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, IHC, WB

Conjugation

Unconjugated

CloneNo.

230214D11
-_- / -_-

Cat No. 82943-1-RR, 82943-1-PBS
PDGFR beta Polyclonal antibody

KO/KD Validated

40 Publications

Reviews (1)

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mouse lung tissue were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Sizes
-_- / -_-

Cat No. 13449-1-AP
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mouse lung tissue were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PDGFR alpha Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP01256-1	84383-5-PBS	98141-1-PBS	PDGFR alpha	Human	Cytometric bead array	CBA: 0.781-100 ng/mL
MP01256-2	84383-2-PBS	84383-3-PBS	PDGFR alpha	Human	Cytometric bead array	CBA: 0.781-100 ng/mL
MP01256-3	84383-4-PBS	84383-1-PBS	PDGFR alpha	Human	Cytometric bead array	CBA: 0.781-100 ng/mL

PDGFR beta Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00006-1	82943-2-PBS	82943-4-PBS	PDGFR beta	human	Cytometric bead array	CBA: 0.313-10 ng/mL
MP00006-2	82943-5-PBS	82943-4-PBS	PDGFR beta	human	Cytometric bead array	CBA: 1.25-20 ng/mL
MP00006-3	82943-6-PBS	82943-3-PBS	PDGFR beta	human	Sandwich ELISA	sELISA: 0.156-10 ng/mL
MP00006-4	82943-7-PBS	82943-3-PBS	PDGFR beta	human	Sandwich ELISA	sELISA: 0.156-10 ng/mL
MP50756-1	60535-1-PBS	60535-2-PBS	PDGFR beta	Human	Cytometric bead array	CBA: 0.098-100 ng/mL
MP50756-2	60535-3-PBS	60535-4-PBS	PDGFR beta	Human	Cytometric bead array	CBA: 0.098-100 ng/mL
MP50756-3	60535-5-PBS	60535-6-PBS	PDGFR beta	Human	Cytometric bead array	CBA: 0.098-100 ng/mL
MP50756-4	60535-7-PBS	60535-8-PBS	PDGFR beta	Human	Cytometric bead array	CBA: 0.391-100 ng/mL
MP50756-5	60542-1-PBS	60543-1-PBS	PDGFR beta	Human	Cytometric bead array	CBA: 3.125-200 ng/mL

PDGFRL Polyclonal antibody

3 Publications

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L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 16217-1-AP
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L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).

Immunofluorescent analysis of L02 cells using 16217-1-AP (PDGFRL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PDAP1 Polyclonal antibodies (2 total products)

2 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 15081-1-AP, CL488-15081

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human PDGFbb protein

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HumanKine® recombinant human PDGFaa protein

-_- / -_-

HumanKine® recombinant human PDGFbb protein- GMP grade

-_- / -_-

PDGFB Recombinant antibodies (230486A7) (2 total products)

-_- / -_-

PDGF-D Polyclonal antibodies (2 total products)

-_- / -_-

PDGFB Matched Antibody PairsNew

Human PDGF-BB ELISA Kit

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NeutraKine® PDGF-BB Monoclonal antibody

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NeutraControl PDGF-BB Monoclonal antibody

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Human PDGF-AB ELISA Kit

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PDGFA Polyclonal antibody

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PDGFC Polyclonal antibody

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PDGFA E.Coli expressed Tagged-proteins (3 total products)

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PDGFB E.Coli expressed Tagged-proteins (2 total products)

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PDGFD E.Coli expressed Tagged-proteins (3 total products)

-_- / -_-

PDGFR beta Recombinant antibodies (230214D11) (2 total products)

-_- / -_-

PDGFR beta Polyclonal antibody

-_- / -_-

PDGFR alpha Matched Antibody PairsNew

PDGFR beta Matched Antibody PairsNew

PDGFRL Polyclonal antibody

-_- / -_-

PDAP1 Polyclonal antibodies (2 total products)

-_- / -_-

Products derived from

PDGFB Matched Antibody Pairs^New

PDGFR alpha Matched Antibody Pairs^New

PDGFR beta Matched Antibody Pairs^New