Results

366 product results for "pyroptosis"

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HumanKine® recombinant human TNF alpha protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

52 Publications

Reviews (2)

View More Images (3)

×
Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.002-0.026 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1014
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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL. 

Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.
HumanKine® recombinant human IL-1 beta protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

41 Publications

View More Images (3)

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Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.8-4.0 ng/mL in an HEK293 reporter cell line, Typically ≤ 0.05 ng/mL in D10.G4.1 cells

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1164
×

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Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.  

Recombinant human IL-1 beta (Cat no: HZ-1164) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.8-4.0 ng/mL ng/mL EC50. 
HumanKine® recombinant human MCP-1 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

View More Images (2)

×
Purity of recombinant human MCP-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

150-750 ng/mL

Expression

HEK293

Reactivity

human

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1334
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Purity of recombinant human MCP-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human MCP-1 (HZ-1334) stimulates dose-dependent proliferation of THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant MCP-1 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 150-750 ng/mL.
HumanKine® recombinant human TNF alpha protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

View More Images (2)

×
Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.002-0.026 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1014-GMP
×

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Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human TNF alpha (HZ-1014-GMP) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.002-0.026 ng/mL.
Cleaved Caspase 3 Recombinant antibodies (230527B2) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

230527B2
-_- / -_-

Cat No. 82707-13-RR, 82707-13-PBS
Cleaved Caspase 3; p17 Monoclonal antibodies (2F7B8) (2 total products)

5 Publications

Reviews (1)

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat

Applications

ELISA, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

Unconjugated

CloneNo.

2F7B8
-_- / -_-

Cat No. 68773-1-Ig, 68773-1-PBS
Cleaved Caspase 3 Polyclonal antibody

118 Publications

View More Images (3)

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Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, chicken, goat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 25128-1-AP
×

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Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (25128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Cleaved Caspase 3 Recombinant antibody, PBS Only (Capture)

Enlarge Image

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Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

CloneNo.

230527B9

Sizes
-_- / -_-

Cat No. 82707-8-PBS
×

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Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.
Cleaved Caspase 3 Recombinant antibody, PBS Only (Capture)

View More Images (2)

×
Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

CloneNo.

230527G6

Sizes
-_- / -_-

Cat No. 82707-10-PBS
×

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Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.
Cleaved Caspase 3 Recombinant antibody, PBS Only (Detector)

View More Images (2)

×
Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

CloneNo.

230527H5

Sizes
-_- / -_-

Cat No. 82707-11-PBS
×

The search results gallery shows a selection of our total image library
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Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.
Cleaved Caspase 3 Recombinant antibody, PBS Only (Detector)

Enlarge Image

×
Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

CloneNo.

230527F3

Sizes
-_- / -_-

Cat No. 82707-9-PBS
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.
Caspase 3/p17/p19 Polyclonal antibodies (3 total products)

KO/KD Validated

2229 Publications

Reviews (8)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, goat, hamster, monkey, rabbit, sheep, zebrafish

Applications

FC (Intra), IF/ICC, IF-P, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated
-_- / -_-

Cat No. CL488-19677, 19677-1-AP, CL594-19677
NF-κB p65 Polyclonal antibodies (4 total products)

KO/KD Validated

1415 Publications

Reviews (7)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, fish, goat, hamster, monkey, pig, rabbit, sheep

Applications

ChIP, CoIP, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®594, Unconjugated
-_- / -_-

Cat No. CL594-10745, CL488-10745, 10745-1-AP, CL647-10745
Caspase 1/p20/p10 Polyclonal antibody

KO/KD Validated

839 Publications

Reviews (2)

View More Images (7)

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Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 10x lens).

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, canine, chicken, hamster, monkey, pig

Applications

WB, IHC, IF, IP, ELISA

Sizes
-_- / -_-

Cat No. 22915-1-AP
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Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

WB result of Caspase 1/p20/p10 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells.

IF result of 22915-1-AP from publication with PMID: 31611559.
NLRP3 Polyclonal antibody

57 Publications

View More Images (5)

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Various lysates were subjected to SDS PAGE followed by western blot with 27458-1-AP (NLRP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Sizes
-_- / -_-

Cat No. 27458-1-AP
×

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Various lysates were subjected to SDS PAGE followed by western blot with 27458-1-AP (NLRP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-NLRP3 (IP:27458-1-AP, 4ug; Detection:27458-1-AP 1:800) with THP-1 cells lysate 1520 ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27458-1-AP (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27458-1-AP (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (27458-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IL-1 Beta Polyclonal antibody

KO/KD Validated

721 Publications

Reviews (2)

View More Images (3)

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Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, canine, pig, rabbit, sheep, zebrafish

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 16806-1-AP
×

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Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
TNF-alpha Polyclonal antibody

674 Publications

Reviews (2)

Enlarge Image

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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, fish, rabbit, sheep

Applications

WB, ELISA, Cell treatment, Transwell Assay

Sizes
-_- / -_-

Cat No. 17590-1-AP
×

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Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
TLR4 Polyclonal antibody

KO/KD Validated

519 Publications

Reviews (1)

View More Images (3)

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mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, canine, chicken, hamster, pig, rabbit

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA, Microtiter plate binding assay

Sizes
-_- / -_-

Cat No. 19811-1-AP
×

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mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).
IkB Alpha Polyclonal antibodies (3 total products)

509 Publications

Reviews (2)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, chicken, ducks, pig

Applications

CoIP, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, Unconjugated
-_- / -_-

Cat No. CL488-10268, CL647-10268, 10268-1-AP
NLRP3 Polyclonal antibodies (3 total products)

KO/KD Validated

500 Publications

Reviews (3)

Host/IsoType

Rabbit/IgG

Reactivity

human, bovine, goat, pig

Applications

CoIP, FC (Intra), IF/ICC, IHC, IP, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated
-_- / -_-

Cat No. 19771-1-AP, CL594-19771, CL488-19771
TNF-alpha Monoclonal antibodies (7B8A11) (4 total products)

KO/KD Validated

471 Publications

Reviews (2)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, bovine, chicken, duck, monkey, pig, rabbit

Applications

ELISA, IF-P, IHC, RIP, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

7B8A11
-_- / -_-

Cat No. CL594-60291, 60291-1-Ig, 60291-1-PBS, CL488-60291

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human TNF alpha protein

-_- / -_-

HumanKine® recombinant human IL-1 beta protein

-_- / -_-

HumanKine® recombinant human MCP-1 protein

-_- / -_-

HumanKine® recombinant human TNF alpha protein- GMP grade

-_- / -_-

Cleaved Caspase 3 Recombinant antibodies (230527B2) (2 total products)

-_- / -_-

Cleaved Caspase 3; p17 Monoclonal antibodies (2F7B8) (2 total products)

-_- / -_-

Cleaved Caspase 3 Polyclonal antibody

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Cleaved Caspase 3 Recombinant antibody, PBS Only (Capture)

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Cleaved Caspase 3 Recombinant antibody, PBS Only (Capture)

-_- / -_-

Cleaved Caspase 3 Recombinant antibody, PBS Only (Detector)

-_- / -_-

Cleaved Caspase 3 Recombinant antibody, PBS Only (Detector)

-_- / -_-

Caspase 3/p17/p19 Polyclonal antibodies (3 total products)

-_- / -_-

NF-κB p65 Polyclonal antibodies (4 total products)

-_- / -_-

Caspase 1/p20/p10 Polyclonal antibody

-_- / -_-

NLRP3 Polyclonal antibody

-_- / -_-

IL-1 Beta Polyclonal antibody

-_- / -_-

TNF-alpha Polyclonal antibody

-_- / -_-

TLR4 Polyclonal antibody

-_- / -_-

IkB Alpha Polyclonal antibodies (3 total products)

-_- / -_-

NLRP3 Polyclonal antibodies (3 total products)

-_- / -_-

TNF-alpha Monoclonal antibodies (7B8A11) (4 total products)

-_- / -_-

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