Results

HumanKine® recombinant human TGF beta 1 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

187 Publications

Reviews (3)

View More Images (3)

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Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Activity

Typically ≤ 0.5 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat, goat, pig

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1011
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Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL. 

Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)
HumanKine® recombinant human TGF beta 1 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

1 Publications

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Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Activity

0.01-0.17 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1011-GMP
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Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.
TGF Beta 1 Recombinant antibodies (230544B7) (3 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 750, Unconjugated

CloneNo.

230544B7
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Cat No. 81746-2-RR, 81746-2-PBS, CL750-81746-2
TGF Beta 1 Polyclonal antibody

KO/KD Validated

660 Publications

Reviews (8)

View More Images (3)

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Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, canine, duck, pig, rabbit, zebrafish

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Sizes
-_- / -_-

Cat No. 21898-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
NeutraKine® TGF beta 1 Monoclonal antibody

6 Publications

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Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

IHC, Neutralization, ELISA

CloneNo.

1E3E9

Sizes
-_- / -_-

Cat No. 69012-1-Ig
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Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Human TGF-beta1 ELISA Kit

49 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants, Urine

Sensitivity

3.3 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE00002
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Rat TGF-beta1 ELISA Kit

10 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

3.4 pg/mL

Range

31.25 - 2000 pg/mL

Sizes
-_- / -_-

Cat No. KE20010
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IHCeasy TGF beta 1 Ready-To-Use IHC Kit

1 Publications

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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0131 (TGF beta 1 IHC Kit).

Primary Ab Type

Mouse Monoclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC0131
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0131 (TGF beta 1 IHC Kit).
NeutraControl TGF beta 1 Monoclonal antibody

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Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Non-Neutralization

CloneNo.

1D3D11

Sizes
-_- / -_-

Cat No. 69512-1-Ig
×

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More validation images available on the product page

Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
TGFB1 E.Coli expressed Tagged-proteins (3 total products)

Species

human

Source

PET28a, PET30a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag27942, Ag24881, Ag24877
Mouse TGF-beta1 ELISA Kit

43 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

16.0 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE10005
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TGF beta 1 Polyclonal antibody

2 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 26155-1-AP
×

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Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TGF beta 1 antibody (26155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HumanKine® recombinant human TGF beta 1 protein

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HumanKine® recombinant human TGF beta 1 protein- GMP grade

-_- / -_-

TGF Beta 1 Recombinant antibodies (230544B7) (3 total products)

-_- / -_-

TGF Beta 1 Polyclonal antibody

-_- / -_-

NeutraKine® TGF beta 1 Monoclonal antibody

-_- / -_-

Human TGF-beta1 ELISA Kit

-_- / -_-

Rat TGF-beta1 ELISA Kit

-_- / -_-

IHCeasy TGF beta 1 Ready-To-Use IHC Kit

-_- / -_-

NeutraControl TGF beta 1 Monoclonal antibody

-_- / -_-

TGFB1 E.Coli expressed Tagged-proteins (3 total products)

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Mouse TGF-beta1 ELISA Kit

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TGF beta 1 Polyclonal antibody

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