Various lysates were subjected to SDS PAGE followed by western blot with 66452-1-Ig (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.
