V5-Trap® Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-Through, B: Bound.
V5-Trap® Agarose for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 I: Input, FT: Flow-Through, B: Bound.
V5-Trap® Agarose for pull-down. The V5-tag is N-terminal. I: Input, FT: Flow-Through, B: Bound.
IP from different cell lysates without V5-tagged proteins => The V5-Trap® has very low background.
