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118 product results for "vegf"

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HumanKine® recombinant human VEGF165 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

34 Publications

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Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

0.3-3.75 ng/mL

Expression

HEK293

Reactivity

human, pig

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1038
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Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human VEGF165 (HZ-1038) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.
HumanKine® recombinant human VEGF121 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

19 Publications

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Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

Typically ≤ 15 ng/mL

Expression

HEK293

Reactivity

human, mouse, pig

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1204
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Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL. 
HumanKine® recombinant human VEGF165 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

1 Publications

Reviews (1)

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Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.3-3.75 ng/mL

Expression

HEK293

Reactivity

human

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1038-GMP
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Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human VEGF165 (HZ-1038-GMP) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF165 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.

GMP Recombinant human VEGF165 (HZ-1038-GMP) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of GMP recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.
HumanKine® recombinant human VEGF121 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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Purity of GMP recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

1.5-9 ng/mL

Expression

HEK293

Reactivity

human, pig

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1204-GMP
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Purity of GMP recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human GMP VEGF121 (HZ-1204-GMP) induces dose-dependent luciferase production in a HEK293T VEGF luciferase reporter cell line. Luciferase production was assessed by the One-Step™ luciferase assay Kit. HEK293T VEGF luciferase reporter cells were treated with increasing concentrations of GMP recombinant VEGF121 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 1.5-9 ng/mL
VEGFA Recombinant antibodies (7H10) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

ELISA, IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

7H10
-_- / -_-

Cat No. 81323-2-PBS, 81323-2-RR
VEGFA Polyclonal antibodies (3 total products)

KO/KD Validated

777 Publications

Reviews (3)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, goat, monkey, pig, rabbit, sheep

Applications

FC (Intra), IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated
-_- / -_-

Cat No. CL488-19003, CL594-19003, 19003-1-AP
VEGFA Monoclonal antibodies (2E2H9) (4 total products)

KO/KD Validated

110 Publications

Host/IsoType

Mouse/IgG2b

Reactivity

human, rat, goat, pig, zebrafish

Applications

ELISA, FC (Intra), IF/ICC, IF-P, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

2E2H9
-_- / -_-

Cat No. 66828-1-Ig, CL488-66828, CL594-66828, 66828-1-PBS
AuthentiKine™ Human VEGF ELISA Kit

24 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

1 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE00216
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Vegfa Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00561-1	83572-4-PBS	83572-2-PBS	Vegfa	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00561-2	83572-5-PBS	83572-2-PBS	Vegfa	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00561-3	83572-3-PBS	83572-1-PBS	Vegfa	Mouse	Cytometric bead array	CBA: 0.625-80 ng/mL
MP00598-1	83610-2-PBS	83610-1-PBS	Vegfa	Rat	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00598-2	83610-3-PBS	83610-1-PBS	Vegfa	Rat	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00598-3	83610-1-PBS	83610-2-PBS	Vegfa	Rat	Sandwich ELISA	sELISA: 31.25-2000 pg/mL
MP01495-1	84687-1-PBS	84687-2-PBS	VEGFA	Human	Cytometric bead array	CBA: 0.625-80 ng/mL

L-VEGFA Polyclonal antibody

3 Publications

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MCF-7 cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 22341-1-AP
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MCF-7 cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit

1 Publications

Reviews (1)

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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0735 (VEGFA/VEGF IHC Kit).

Primary Ab Type

Mouse Monoclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC0735
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0735 (VEGFA/VEGF IHC Kit).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC0735 (VEGFA/VEGF IHC Kit).
NeutraKine®VEGF165/121 Monoclonal antibody

1 Publications

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Recombinant human VEGF165 (Cat.NO. HZ-1038) stimulates proliferation of HUVEC (human umbilical vein endothelial) cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human VEGF165 (10 ng/mL HZ-1038) is neutralized by mouse anti-human VEGF165/121 monoclonal antibody 69025-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-2 μg/mL. The NeutraControl mouse anti-human VEGF165 antibody 69525-1-Ig could recognize human VEGF165 but could not neutralize human VEGF165 acitivity in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Neutralization

CloneNo.

6C3D5

Sizes
-_- / -_-

Cat No. 69025-1-Ig
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Recombinant human VEGF165 (Cat.NO. HZ-1038) stimulates proliferation of HUVEC (human umbilical vein endothelial) cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human VEGF165 (10 ng/mL HZ-1038) is neutralized by mouse anti-human VEGF165/121 monoclonal antibody 69025-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-2 μg/mL. The NeutraControl mouse anti-human VEGF165 antibody 69525-1-Ig could recognize human VEGF165 but could not neutralize human VEGF165 acitivity in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human VEGF165 (Cat.NO. HZ-1038) and VEGF121 (Cat.NO. HZ-1204) respectively at 70 ng/well followed by blocking and adding serial diluted VEGF165/121 antibody 69025-1-Ig and VEGF165 antibody 69525-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggsts that 69025-1-Ig recognizes both VEGF165 and VEGF121 while 69525-1-Ig recognize VEGF165 only.
NeutraControl VEGF165 Monoclonal antibody

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Recombinant human VEGF165 (Cat.NO. HZ-1038) stimulates proliferation of HUVEC (human umbilical vein endothelial) cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human VEGF165 (10 ng/mL HZ-1038) is neutralized by mouse anti-human VEGF165/121 monoclonal antibody 69025-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-2 μg/mL. The NeutraControl mouse anti-human VEGF165 antibody 69525-1-Ig could recognize human VEGF165 but could not neutralize human VEGF165 acitivity in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, Non-Neutralization

CloneNo.

7E9F4

Sizes
-_- / -_-

Cat No. 69525-1-Ig
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Recombinant human VEGF165 (Cat.NO. HZ-1038) stimulates proliferation of HUVEC (human umbilical vein endothelial) cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human VEGF165 (10 ng/mL HZ-1038) is neutralized by mouse anti-human VEGF165/121 monoclonal antibody 69025-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-2 μg/mL. The NeutraControl mouse anti-human VEGF165 antibody 69525-1-Ig could recognize human VEGF165 but could not neutralize human VEGF165 acitivity in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human VEGF165 (Cat.NO. HZ-1038) and VEGF121 (Cat.NO. HZ-1204) respectively at 70 ng/well followed by blocking and adding serial diluted VEGF165/121 antibody 69025-1-Ig and VEGF165 antibody 69525-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggsts that 69025-1-Ig recognizes both VEGF165 and VEGF121 while 69525-1-Ig recognize VEGF165 only.
VEGFA Recombinant antibodies (1G5) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

1G5
-_- / -_-

Cat No. 82864-16-PBS, 82864-16-RR
Mouse VEGF ELISA Kit

12 Publications

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

2.0 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE10009
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Vegfa Polyclonal antibody

9 Publications

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Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 10x lens).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 26157-1-AP
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 40x lens).

mouse brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Rat VEGF ELISA Kit

5 Publications

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Assay Type

Sandwich

Sample Type

Cell culture supernates

Sensitivity

6.7 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE20014
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Rat VEGF ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

2.1 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE20027
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Standard Curve of KE20027 Rat VEGF ELISA Kit
VEGFA E.Coli expressed Tagged-proteins (3 total products)

2 Publications

Species

human

Source

PET28a, PET30a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag24720, Ag13500, Ag24740
Vegfa Recombinant antibodies (2D13) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

ELISA, IF/ICC

Conjugation

Unconjugated

CloneNo.

2D13
-_- / -_-

Cat No. 82864-9-PBS, 82864-9-RR
Vegfa Recombinant antibodies (2I18) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

ELISA, IF/ICC

Conjugation

Unconjugated

CloneNo.

2I18
-_- / -_-

Cat No. 82864-7-RR, 82864-7-PBS