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47 product results for "wnt"

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HumanKine® recombinant human Wnt3A protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

4 Publications

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Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Activity

25-500 ng/mL in reporter cells

Expression

HEK293

Reactivity

human, mouse

Purity

>90%

Sizes
-_- / -_-

Cat No. HZ-1296
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Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human Wnt3A (HZ-1296) induces dose-dependent alkaline phosphatase production in MC3T3 E1 mouse preosteoblast cells using pNPP as chromogenic substrate. MC3T3 E1 cells were treated with increasing concentrations of recombinant Wnt3A for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-17 ng/mL.

Recombinant human Wnt3A (HZ-1296) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

Proteintech Wnt3A (HZ-1296) demonstrates a 3-fold lower EC50 and greater induction of luciferase production than a leading competitor. Recombinant human Wnt3A induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence
HumanKine® recombinant human Wnt3A protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

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×
Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

25-500 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>90%

Sizes
-_- / -_-

Cat No. HZ-1296-GMP
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Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human GMP Wnt3A (HZ-1296-GMP) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of GMP recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 25-500 ng/mL

GMP Recombinant human GMP Wnt3A (HZ-1296-GMP) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of GMP recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 25-500 ng/mL.
WNT5A/B Polyclonal antibody

KO/KD Validated

53 Publications

Reviews (1)

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mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, zebrafish

Applications

WB, IHC, IF, IP, RIP, ELISA

Sizes
-_- / -_-

Cat No. 55184-1-AP
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mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WNT3A Polyclonal antibody

31 Publications

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fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 26744-1-AP
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fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WNT7A/B Polyclonal antibody

KO/KD Validated

11 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Sizes
-_- / -_-

Cat No. 10605-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-WNT7A (IP:10605-1-AP, 4ug; Detection:10605-1-AP 1:4000) with mouse kidney tissue lysate 2280 ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (10605-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

WNT3A Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP01423-1	84577-3-PBS	84577-1-PBS	WNT3A	Human	Cytometric bead array	CBA: 0.781-100 ng/mL
MP01423-2	84577-3-PBS	84577-2-PBS	WNT3A	Human	Cytometric bead array	CBA: 0.781-100 ng/mL

WNT10B Monoclonal antibodies (1G11E4) (3 total products)

KO/KD Validated

4 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse

Applications

ELISA, FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

1G11E4
-_- / -_-

Cat No. CL488-67210, 67210-1-PBS, 67210-1-Ig
WNT5A/B Polyclonal antibody

3 Publications

Reviews (1)

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Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 29793-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IHCeasy WNT10B Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC2051 (WNT10B IHC Kit).

Primary Ab Type

Mouse Monoclonal

Reactivity

human, mouse

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC2051
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC2051 (WNT10B IHC Kit).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC2051 (WNT10B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC2051 (WNT10B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using KHC2051 (WNT10B IHC Kit).
WNT7A Polyclonal antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 27177-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
WNT8A Polyclonal antibodies (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human, rat

Applications

IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. CL488-30518, 30518-1-AP
WNT3A E.Coli expressed Tagged-proteins (4 total products)

2 Publications

Species

human

Source

PET28a, PET30a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag25047, Ag25051, Ag17232, Ag15834
WNT10B E.Coli expressed Tagged-proteins (2 total products)

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
-_- / -_-

Cat No. Ag17114, Ag24180
WNT7A Fusion Protein

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Species

human

Source

E. coli-derived, PET28a

Tag

6*His

Format

Powder

Sizes
-_- / -_-

Cat No. Ag26085
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WNT7A fusion protein Ag26085 SDS-PAGE
WNT1 Polyclonal antibody

KO/KD Validated

41 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Sizes
-_- / -_-

Cat No. 27935-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human WNT1 (27935-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WNT2 Polyclonal antibodies (2 total products)

KO/KD Validated

12 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC, IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. 27214-1-AP, CL488-27214
WNT4 Polyclonal antibody

9 Publications

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Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 14371-1-AP
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Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 14371-1-AP (WNT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
WNT6 Polyclonal antibody

KO/KD Validated

8 Publications

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SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, chicken

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 24201-1-AP
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SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WNT2 Monoclonal antibodies (3H7B10) (2 total products)

KO/KD Validated

6 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat

Applications

ELISA, IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

3H7B10
-_- / -_-

Cat No. 66656-1-Ig, 66656-1-PBS
WNT10A Polyclonal antibody

4 Publications

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Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, goat

Applications

WB, IHC, IF/ICC, ELISA

Sizes
-_- / -_-

Cat No. 26238-1-AP
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Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 40x lens).
WNT16 Polyclonal antibody

3 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 28610-1-AP (WNT16 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Sizes
-_- / -_-

Cat No. 28610-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 28610-1-AP (WNT16 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.