HRP-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, zebrafish, plant and More (2)

Applications

WB, IF, ELISA

Conjugate

HRP Fluorescent Dye

CloneNo.

7D2C10

Cat no : HRP-60008

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP β actin, HRP-ACTB, HRP-Beta actin, HRP-F actin, PS1TP5BP1, β actin



Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Sample-dependent, check data in validation data gallery

Product Information

HRP-60008 targets Beta Actin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivitycanine, hamster, human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

SpeciesApplicationTitle
mouseWB

Am J Transl Res

Isoliquiritigenin attenuates LPS-induced AKI by suppression of inflammation involving NF-κB pathway.

Authors - Yun Tang
humanWB

Am J Cancer Res

Dysregulated ENPP1 increases the malignancy of human lung cancer by inducing epithelial-mesenchymal transition phenotypes and stem cell features.

Authors - Min Hu
mouseWB

Oncol Rep

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

Authors - Yifan Li
humanWB

Autophagy

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Authors - Xing Huang
humanWB

Cell Death Dis

Iron-dependent CDK1 activity promotes lung carcinogenesis via activation of the GP130/STAT3 signaling pathway.

Authors - Yanbin Kuang
humanWB

Exp Ther Med

Downregulation of nucleolar and spindle-associated protein 1 expression suppresses liver cancer cell function.

Authors - Yifan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (06-07-2021)

The antibody works well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: NSC34 cells
HRP Beta Actin Antibody Western Blot validation (1:10000 dilution) in NSC34 cells (Cat no:HRP-60008)
FH

Hasibur (Verified Customer) (08-01-2019)

Nice antibody and no need for secondary antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: Bladder Cancer
FH

Joanne (Verified Customer) (01-22-2019)

Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: caco-2 cell
HRP Beta Actin Antibody Western Blot, validation (1:5000 dilution) in caco-2 cell (Cat no:HRP-60008)
FH

Tess (Verified Customer) (11-29-2018)

Little to no background, and takes half the time.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: human colorectal cancer cell line
HRP Beta Actin Antibody Western Blot, validation (1:10,000 dilution) in human colorectal cancer cell line (Cat no:HRP-60008)