Beta Actin Monoclonal antibody

Used for Western Blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Used for WB, Highly Recommended

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

Used on Simple Western (CE-SDS); Highly Recommended!

• Applications: Other
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Rhesus Macaque Brain

Used for WB, excellent product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cell line

Used for western blotting , very nice product. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cell line

• Applications: Western Blot
• Primary Antibody Dilution: 3000
• Cell Tissue Type: l2

Worked really well when loading 30ug protein and incubating for 1.5h at RT. Clear signal and single band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Ewing sarcoma cells

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 3000
• Cell Tissue Type: AC16

Could not detect B-actin in the sample although MS analysis confirmed it was there.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: Dorsal Root Ganglion, Spinal Cord

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hek293, nsc cells

The beta actin works very well for 40ug total protein loaded

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: HEK293T cells

Excellent antibody, can be re-used multiple times without reduction in signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: 293T

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

Separating Gel: 10% poly-acrylamide Transfer at 400mA for 2hr onto nitrocellulose membrane at 4oC Blocking and antibodies dilutions: 5% non-fat milk in PBS-Tween20 for 1 hr at RT Incubation with primary Abs: overnight at 4oC Incubation with secondary Abs: 1hr at 4oC

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto

Everything was fine

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Ovarian cancer cell lines

Excellent antibody, can be re-used multiple times without reduction in signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Fibroblasts

Works good for Elisa assay

• Applications: Other
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: hela

I will recommend this antibody for Immunoblotting. it is working ar 1:6000 dilution. Even it will work at 1:8000 dilution but I just used at 1:6000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:6000
• Cell Tissue Type: colon tissue and HCT116 cells, SW620 cell

This B-Actin antibody works excellently. The dilution also significantly high. I use 1:20,000 and this works great for me.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: For all invitro western blot housekeeping marker

This B-Actin antibody works excellently. The dilution also significantly high. I use 1:20,000 and this works great for me.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: For all invitro western blot housekeeping marker

A very good and convincing antibody to detect beta-actin in mouse tissues and various cell lines lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:15000
• Cell Tissue Type: mouse cortex lysate

Very good signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Colon cancer cells

Very specific antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000-10000
• Cell Tissue Type: mouse BMDM

Very good and strong staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: SW1353 chondrosarcoma

We ordered this actin antibody to detect actin as internal control in the cell lysates during the western blot, and the antibody is very sensitive.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

Consistently works antibody

• Applications: Western Blot

The Beta Actin antibody works fine.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

We ordered this actin antibody to detect actin in the cell lysates during the western blot, and it works very well, and the antibody is very sensitive. We highly recommend it.

• Applications: Western Blot
• Cell Tissue Type: human macrophages

This antibody work really well in cells and tissues (mouse). I have also used in human skeletal muscle with good results as well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: C2C12, Heart, skeletal muscle, adipose, kidney, liver

Works nicely.Used on Human brain lysates (temporal cortex).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human brain lysate

Very good product; signal is good

• Applications: Western Blot

Generate very consistent result for my porcine brain endothelial cells. The antibody at dilution of 1:5000 can be reused for multiple times.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: porcine brain endothelial cells

I needed to run a housekeeping gene control for my protein expression test. I was testing HEK293T cell lysates. My Lab did not have a proper AB, but a colleague from a lab next door borrowed me a sample from Proteintech. I was very happy with the results, they were consistent among my triplicate. I would purchase this AB but as a HRP conjugate, as it saves more time and provides cleaner results.

• Applications: Western Blot,
• Cell Tissue Type: HEK293T

The antibody works very nicely, very clean and I used only 10 uL of protein.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Cell line C2C12

I used this antibody for Hydra actin immunostaining. The outcome is quite good.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500

Very specific and strong labeling in whole cell lysates.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Cos-7

Very good anitbody. Works well in Western for various type of cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Primary cells, various cell lines

in the testing staining it kind of worked, even if with higher dilution it could work better.anyway further test should be done to confirm it.if results are reproducible it might be a valid alternative to more expensive antibody

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/1500
• Cell Tissue Type: Brain cells

This antibody works very well for Western blot using vascular tissue or cell lysates.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: aorta