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Beta Actin 抗体 2854 文献

Mouse Monoclonal| カタログ番号: 66009-1-Ig |クローン番号: 2D4H5

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
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Images See all

Beta Actin Antibody IF MDCK cells 66009-1-Ig

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Beta Actin Antibody WB A549 cells 66009-1-Ig

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

Beta Actin Antibody IP HeLa cells 66009-1-Ig

IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

Beta Actin Antibody FC HeLa cells 66009-1-Ig

1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Beta Actin Antibody IHC human kidney tissue 66009-1-Ig

Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).

Beta Actin Antibody WB HeLa cells 66009-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

種特異性:
human, mouse, rat, hamster, zebrafish, monkey, dog, A.fumigatus strains, Aedes albopictus, Arabidopsis, bovine, canine, chicken, Cyprinus carpio, Drosophila, duck
WB ポジティブ:
HeLa cells, Jurkat cells, A549 cells, HEK-293 cells, HepG2 cells, K-562 cells, HHSC-T6 cells, NIH/3T3 cells, 4T1 cells, CHO cells, mouse pancreas, rat brain, rat pancreas
IP ポジティブ:
HeLa cells
IHC ポジティブ:
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue
IF ポジティブ:
MDCK cells, HeLa cells
FC ポジティブ:
HeLa cells
推奨希釈倍率:
WB : 1:20000-1:100000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:20-1:2000
IF : 1:500-1:2000

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG2b
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Fusion Protein
正式名:
actin, beta
計算分子量:
42 kDa
検出分子量:
42 kDa
GenBank accession number:
NM_001101
Gene ID (NCBI):
60
遺伝子シンボル:
ACTB
別名:
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

背景説明

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.


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