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- KD/KO Validated
CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, canine, monkey, zebrafish, hamster
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D4H5
Cat no : CL488-66009
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells |
| Positive IF/ICC detected in | MDCK cells, U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 3 publications below |
Product Information
CL488-66009 targets Beta Actin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine, monkey, zebrafish, hamster samples.
| Tested Reactivity | human, mouse, rat, canine, monkey, zebrafish, hamster |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | actin, beta |
| Calculated molecular weight | 42 kDa |
| GenBank accession number | NM_001101 |
| Gene symbol | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2883225 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 Beta Actin antibody CL488-66009 | Download protocol |
| IF protocol for CL Plus 488 Beta Actin antibody CL488-66009 | Download protocol |
| FC protocol for CL Plus 488 Beta Actin antibody CL488-66009 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Fingolimod ameliorates schizophrenia-like cognitive impairments induced by phencyclidine in male rats | ||
Mediators Inflamm Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2 | ||
Oncol Lett CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process | ||
J Cell Mol Med YTHDF1 promotes gallbladder cancer progression via post-transcriptional regulation of the m6A/UHRF1 axis | ||
Acta Pharmacol Sin Desloratadine ameliorates paclitaxel-induced peripheral neuropathy and hypersensitivity reactions in mice | ||
Nanoscale Effects of hydrophilic fullerene nanoarchitectured structures on the behaviour of neural stem cells. |
