NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF-P, FC (Intra), Dot blot, ELISA

Conjugate

Unconjugated

Cat no : 26975-1-AP

Synonyms

RBFOX3, Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3


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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive FC (Intra) detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26975-1-AP targets NeuN in WB, IHC, IF-P, FC (Intra), Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig, monkey, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NeuN fusion protein Ag25689 相同性解析による交差性が予測される生物種
Full Name hexaribonucleotide binding protein 3
Observed molecular weight 46-52 kDa
GenBank accession numberNM_001082575
Gene symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2880708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).


Protocols

Product Specific Protocols
WB protocol for NeuN antibody 26975-1-APDownload protocol
IHC protocol for NeuN antibody 26975-1-APDownload protocol
IF protocol for NeuN antibody 26975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Immunity

Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury

Authors - Yangning Lan
mouseIF

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseIF

Sci Adv

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Authors - Sefan Asamitsu
zebrafishIHC

Nat Commun

An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish.

Authors - Chun-Xiao Huang
mouseIHC

Brain

Huntingtin silencing delays onset and slows progression of Huntington's disease: a biomarker study.

Authors - Hongshuai Liu
mouseIHC

Front Behav Neurosci

Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice

Authors - Ying-Ke Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Raquel (Verified Customer) (07-12-2024)

Nice and clean staining. Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs using 26975-1-AP NeuN antibody at dilution of 1:250 (under 20x lens). (Magenta: NeuN ; Blue: DAPI)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Human Brain organoids
NeuN Antibody Immunofluorescence validation (1:250 dilution) in Human Brain organoids (Cat no:26975-1-AP)
FH

Manohar (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neuronal stem cells
FH

Damien (Verified Customer) (02-15-2024)

Works well in 2D staining of induced neurons, starting at 14days. Fixed and permeabilized with 3%BSA and 0.1% Triton

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Induced neurons
FH

Monika (Verified Customer) (06-08-2023)

Very specific and strong signal. Used without antigen retrieval. Worked good for both NBT/BCIP-colorimetry and Alexa fluor-fluorescence detection.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse brain sections (Paraffin embedded)
FH

Russell (Verified Customer) (05-23-2023)

Antigen heat retrieval with Citrate buffer pH 6.0 at 100C for 20 min Permeabilized with 1% TX-100 Blocking Buffer PBST with 5% Donkey Serum

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Brain Organoid
FH

Irina (Verified Customer) (11-18-2021)

Good specific neuronal signal. Works nicely for double IF to look at co-localisation with other proteins.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: FFPE Human brain
FH

David (Verified Customer) (01-14-2020)

Good nuclear localisation, with strong signal and low background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y
FH

Maxence (Verified Customer) (09-05-2019)

Labeling was neat without any background. Signal was quite strong and stable.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Brain
FH

Elena (Verified Customer) (07-31-2019)

Good antibody, stained in all cell lines we used it for.

  • Applications: Western Blot,
  • Cell Tissue Type: HeLA cells, hESCs
FH

Kaspar (Verified Customer) (01-27-2018)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mouse