CoraLite®594-conjugated NeuN Polyclonal antibody
NeuN Polyclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-26975
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse brain tissue, rat brain tissue |
Positive FC (Intra) detected in | U-87 MG cells |
Positive FC detected in | U-87 MG cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-26975 targets NeuN in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NeuN fusion protein Ag25689 相同性解析による交差性が予測される生物種 |
Full Name | hexaribonucleotide binding protein 3 |
Observed molecular weight | 46-52 kDa |
GenBank accession number | NM_001082575 |
Gene symbol | NeuN |
Gene ID (NCBI) | 146713 |
RRID | AB_2919878 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Function
NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).
Tissue specificity
NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).
Involvement in disease
· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).
Isoforms
There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).
Post-translational modifications
Currently not known.
Cellular localization
NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 NeuN antibody CL594-26975 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |