POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15278-1-AP

Synonyms

Arsenic transactivated protein, AsTP, CHARAC17, CHRAC 17, CHRAC17, DNA polymerase II subunit 3, HuCHRAC17, p17, POLE3, YBL1



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

15278-1-AP targets POLE3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLE3 fusion protein Ag7396 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), epsilon 3 (p17 subunit)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC003166
Gene symbol POLE3
Gene ID (NCBI) 54107
RRIDAB_2166751
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLE3 antibody 15278-1-APDownload protocol
IHC protocol for POLE3 antibody 15278-1-APDownload protocol
IF protocol for POLE3 antibody 15278-1-APDownload protocol
IP protocol for POLE3 antibody 15278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.

Authors - Chuanyou Yi
humanWB

Front Oncol

ASF1B Promotes Oncogenesis in Lung Adenocarcinoma and Other Cancer Types.

Authors - Wencheng Zhang