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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated POLE3 Polyclonal ant (CL488-15278)

    IF Staining of HepG2 using CL488-15278

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 POLE3 antibody (CL488-15278) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for IF/ICC
Cat No. CL488-15278

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Arsenic transactivated protein, AsTP, CHARAC17, CHRAC 17, CHRAC17, DNA polymerase II subunit 3, HuCHRAC17, p17, POLE3, YBL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15278 targets POLE3 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLE3 fusion protein Ag7396 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), epsilon 3 (p17 subunit)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC003166
Gene Symbol POLE3
Gene ID (NCBI) 54107
RRIDAB_3672628
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRF9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 POLE3 antibody CL488-15278Download protocol
Standard Protocols
Click here to view our Standard Protocols