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PYCR2 Polyclonal antibody

Name: PYCR2 Polyclonal antibody
Brand: Proteintech
SKU: 17146-1-AP
Rating: 4.75 (4 reviews)

PYCR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (4)

Cat no : 17146-1-AP

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 17146-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17146-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17146-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17146-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17146-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 17146-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 17146-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, L02 cells, Jurkat cells
Positive IHC detected in	human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

17146-1-AP targets PYCR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, african green monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PYCR2 fusion protein Ag10918 相同性解析による交差性が予測される生物種
Full Name	pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight	320 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC020553
Gene symbol	PYCR2
Gene ID (NCBI)	29920
RRID	AB_2253344
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR2 antibody 17146-1-AP	Download protocol
IHC protocol for PYCR2 antibody 17146-1-AP	Download protocol
IF protocol for PYCR2 antibody 17146-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Authors - Qiang Gao View Article
human	WB	Sci Adv Loss of attachment promotes proline accumulation and excretion in cancer cells Authors - Steven E Pilley View Article
mouse	WB	EMBO J Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress. Authors - Simon Schwörer View Article
human	WB	EMBO J Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis. Authors - Zhen Chen View Article
human	WB	Cell Rep Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis. Authors - Kate E R Hollinshead KD Validated View Article
African green monkey	WB	Virol Sin African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2. Authors - Sheng Chen KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH balawant (Verified Customer) (12-25-2023)	I have used this antibody . This antibody is working great. Applications: Western Blot, Immunohistochemistry Primary Antibody Dilution: 1:1000 for western blot and 1:700 for IHC Cell Tissue Type: colon tissue of mice and human
FH Balawant (Verified Customer) (08-06-2022)	This antibody is working great. I have used this antibody for tissue lysate and cell lysate. it has no background. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: colon tissue and colon cancer cells
FH Susmita (Verified Customer) (03-11-2022)	Excellent antibody Applications: Western Blot Primary Antibody Dilution: WB: 1: 2000 Cell Tissue Type: CRC cell line
FH Iram (Verified Customer) (07-03-2020)	Giving a very sharp and clean result Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 17146-1-AP

WB analysis of Jurkat using 17146-1-AP

WB analysis of L02 using 17146-1-AP

WB analysis of Jurkat using 17146-1-AP

WB analysis of HeLa using 17146-1-AP

IHC staining of human breast cancer using 17146-1-AP

IHC staining of human breast cancer using 17146-1-AP

IHC staining of human placenta using 17146-1-AP

IHC staining of human placenta using 17146-1-AP

IHC staining of human lung using 17146-1-AP

IHC staining of human lung using 17146-1-AP

IHC staining of human kidney using 17146-1-AP

IHC staining of human kidney using 17146-1-AP

IHC staining of human testis using 17146-1-AP

IHC staining of human testis using 17146-1-AP

IF Staining of HeLa using 17146-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Loss of attachment promotes proline accumulation and excretion in cancer cells

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.

Reviews