Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 17146-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17146-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17146-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17146-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17146-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 17146-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17146-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, L02 cells, Jurkat cells
Positive IHC detected in	human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 20 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

17146-1-AP targets PYCR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, african green monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10918 Product name: Recombinant human PYCR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC020553 Sequence: MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD 相同性解析による交差性が予測される生物種
Full Name	pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight	320 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC020553
Gene Symbol	PYCR2
Gene ID (NCBI)	29920
RRID	AB_2253344
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96C36
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
IF protocol for PYCR2 antibody 17146-1-AP	Download protocol
IHC protocol for PYCR2 antibody 17146-1-AP	Download protocol
WB protocol for PYCR2 antibody 17146-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Authors - Qiang Gao View Article
human	WB	Sci Adv Loss of attachment promotes proline accumulation and excretion in cancer cells Authors - Steven E Pilley View Article
mouse	WB	EMBO J Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress. Authors - Simon Schwörer View Article
human	WB	EMBO J Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis. Authors - Zhen Chen View Article
human	WB	Cell Rep Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis. Authors - Kate E R Hollinshead KD Validated View Article
African green monkey	WB	Virol Sin African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2. Authors - Sheng Chen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17146-1-AP

WB analysis of L02 using 17146-1-AP

WB analysis of Jurkat using 17146-1-AP

WB analysis of HeLa using 17146-1-AP

WB analysis of Jurkat using 17146-1-AP

IHC staining of human breast cancer using 17146-1-AP

IHC staining of human breast cancer using 17146-1-AP

IHC staining of human testis using 17146-1-AP

IHC staining of human testis using 17146-1-AP

IHC staining of human kidney using 17146-1-AP

IHC staining of human kidney using 17146-1-AP

IHC staining of human placenta using 17146-1-AP

IHC staining of human placenta using 17146-1-AP

IHC staining of human lung using 17146-1-AP

IHC staining of human lung using 17146-1-AP

IF Staining of HeLa using 17146-1-AP

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Loss of attachment promotes proline accumulation and excretion in cancer cells

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.