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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17146

Synonyms

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PYCR2 Polyclonal ant (CL488-17146)

    IF Staining of HeLa using CL488-17146

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PYCR2 antibody (CL488-17146) at dilution of 1:400.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17146 targets PYCR2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR2 fusion protein Ag10918 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight 320 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC020553
Gene symbol PYCR2
Gene ID (NCBI) 29920
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PYCR2 antibody CL488-17146Download protocol
Standard Protocols
Click here to view our Standard Protocols