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RAD51 Monoclonal antibody

RAD51 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4A12B8

Cat no : 67024-1-Ig

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA


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Tested Applications

Positive WB detected inJurkat cells, 4T1 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67024-1-Ig targets RAD51 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD51 fusion protein Ag7410 相同性解析による交差性が予測される生物種
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001459
Gene symbol RAD51
Gene ID (NCBI) 5888
RRIDAB_2882339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Protocols

Product Specific Protocols
WB protocol for RAD51 antibody 67024-1-IgDownload protocol
IHC protocol for RAD51 antibody 67024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Nanobiotechnology

Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles.

Authors - Ruijie Qian
humanWB,IF

Int J Mol Sci

Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer

Authors - Inken Flörkemeier
humanIF

J Med Chem

Rational Design of PARP1/c-Met Dual Inhibitors for Overcoming PARP1 Inhibitor Resistance Induced by c-Met Overexpression

Authors - Zeren Sun

Cell Death Dis

Iron promotes ovarian cancer malignancy and advances platinum resistance by enhancing DNA repair via FTH1/FTL/POLQ/RAD51 axis

Authors - Qingyu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Juri (Verified Customer) (01-02-2024)

We have tested in immunocytochemistry, but did not work. Kindly, proteintech offered a replacement.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 in 200
  • Cell Tissue Type: Cell