RAD51 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

WB result of RAD51 antibody (67024-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

4T1 cells were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.