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RAD51 Monoclonal antibody

RAD51 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67024-1-Ig
Clone No.4A12B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

RAD51 homolog A, HsT16930, HsRad51, HRAD51, BRCC5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, 4T1 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67024-1-Ig targets RAD51 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD51 fusion protein Ag7410 相同性解析による交差性が予測される生物種
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001459
Gene Symbol RAD51
Gene ID (NCBI) 5888
RRIDAB_2882339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Protocols

Product Specific Protocols
WB protocol for RAD51 antibody 67024-1-IgDownload protocol
IHC protocol for RAD51 antibody 67024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Nanobiotechnology

Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles.

Authors - Ruijie Qian
humanWB,IF

Int J Mol Sci

Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer

Authors - Inken Flörkemeier
humanIF

J Med Chem

Rational Design of PARP1/c-Met Dual Inhibitors for Overcoming PARP1 Inhibitor Resistance Induced by c-Met Overexpression

Authors - Zeren Sun

Cell Death Dis

Iron promotes ovarian cancer malignancy and advances platinum resistance by enhancing DNA repair via FTH1/FTL/POLQ/RAD51 axis

Authors - Qingyu Zhang
humanWB,IF

Mol Cancer

NSUN6-mediated 5-methylcytosine modification of NDRG1 mRNA promotes radioresistance in cervical cancer

Authors - Min Yu
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