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KD/KO Validated

SAMHD1 Polyclonal antibody

Name: SAMHD1 Polyclonal antibody
Brand: Proteintech
SKU: 12586-1-AP
Rating: 5.00 (1 reviews)

SAMHD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 12586-1-AP

Synonyms

HDDC1, EC:3.1.5.-, dNTPase, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, Dendritic cell-derived IFNG-induced protein

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 12586-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12586-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12586-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 12586-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12586-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12586-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 12586-1-AP
IP result of anti-SAMHD1 (IP:12586-1-AP, 3ug; Detection:12586-1-AP 1:300) with K-562 cells lysate 2400ug.
IHC staining of human lung cancer using 12586-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12586-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12586-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 12586-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 12586-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (12586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 12586-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (12586-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of K-562 using 12586-1-AP
1x10^6 K-562 cells were intracellularly stained with 0.2 ug SAMHD1 Polyclonal antibody (12586-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 12586-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 16 publications below
WB	See 47 publications below
IHC	See 11 publications below
IF	See 10 publications below
IP	See 3 publications below
FC	See 11 publications below
ChIP	See 2 publications below

Product Information

12586-1-AP targets SAMHD1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SAMHD1 fusion protein Ag3287 相同性解析による交差性が予測される生物種
Full Name	SAM domain and HD domain 1
Calculated molecular weight	626 aa, 72 kDa
Observed molecular weight	64-72 kDa
GenBank accession number	BC036450
Gene symbol	SAMHD1
Gene ID (NCBI)	25939
RRID	AB_2183496
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for SAMHD1 antibody 12586-1-AP	Download protocol
IHC protocol for SAMHD1 antibody 12586-1-AP	Download protocol
IF protocol for SAMHD1 antibody 12586-1-AP	Download protocol
IP protocol for SAMHD1 antibody 12586-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Genet Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response. Authors - Rice Gillian I GI View Article
human	IF	Nat Microbiol Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97. Authors - Ramona Businger View Article
human	WB,IHC	Nat Med PMID: 27991919 Authors - Constanze Schneider View Article
human	WB,IHC	Nat Med PMID: 28067901 Authors - Nikolas Herold View Article
human	WB, IHC, IF	Nat Med SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells. Authors - Baldauf Hanna-Mari HM KD Validated View Article
monkey	WB	Immunity Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells. Authors - Nina Calantone View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Roy (Verified Customer) (06-15-2024)

Great antibody that recognizes specifically SAMHD1 in THP1 cells and MDM, validated through the use of HIV-2 Vpx

Applications: Western Blot
Primary Antibody Dilution: 1/1000
Cell Tissue Type: THP1/MDM

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAMHD1 Polyclonal antibody

SAMHD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 12586-1-AP

WB analysis of human heart using 12586-1-AP

WB analysis of K-562 using 12586-1-AP

WB analysis of L02 using 12586-1-AP

WB analysis of mouse heart using 12586-1-AP

WB analysis of human brain using 12586-1-AP

IP experiment of K-562 using 12586-1-AP

IHC staining of human lung cancer using 12586-1-AP

IHC staining of human lung cancer using 12586-1-AP

IHC staining of human colon cancer using 12586-1-AP

IHC staining of human colon cancer using 12586-1-AP

IF Staining of HepG2 using 12586-1-AP

IF Staining of HepG2 using 12586-1-AP

FC experiment of K-562 using 12586-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.

Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.

PMID: 27991919

PMID: 28067901

SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.

Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells.

Reviews

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